Kit de ensayo de poro de transición MitoProbe™, para citometría de flujo

Citas y referencias (16)

Invitrogen™

Kit de ensayo de poro de transición MitoProbe™, para citometría de flujo

El kit de ensayo de poros de transición MitoProbe™ proporciona un método comprobado (Petronilli V. et al. 1989, 1999) deMás información

Número de catálogo	Cantidad
M34153	1 Kit

Número de catálogo M34153

Precio (MXN)

Cantidad:

1 Kit

El kit de ensayo de poros de transición MitoProbe™ proporciona un método comprobado (Petronilli V. et al. 1989, 1999) de medición de la permeabilidad de la apertura de poros de transición de permeabilidad mitocondrial. El kit incluye una tinción y un desactivador que se cargan fácilmente en las células. En las células sanas, la tinción entra en las mitocondrias, pero no el desactivador. Las mitocondrias permanecen fluorescentes hasta que la activación de los poros mitocondriales permite la desactivación de la fluorescencia.

Consulte la guía de selección para todos los ensayos de apoptosis para citometría de flujo.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Excitación/emisión488

N.º de reacciones100 Reactions

Línea de productosMitoProbe

Tipo de productoKit de ensayo de poros de transición

Cantidad1 Kit

Condiciones de envíoTemperatura ambiente

ConjugadoCalceína AM

FormatoTubo

Unit SizeEach

Contenido y almacenamiento

Contiene 5 viales de calceína, AM (50 µg de polvo seco/vial), 1 vial de cobalto (II) hexahidrato de cloruro (1,2 ml, 80 mM en solución salina), 1 vial de ionomicina (55 µg), sal de calcio y 1 vial de DMSO (1,5 ml).

Almacenar en congelador (de – 5 a – 30 °C).

Preguntas frecuentes

What are the advantages of flow cytometry?

-Measures data from single cells.
-Data are obtained for a large number of cells, generating a rich statistical analysis of cell populations.
-Because single cells are measured, it will reveal heterogeneity within a population.
-With the ability to multiplex, small sub-populations can be identified.
-Thousands of cells can be analyzed rapidly.
-It is ideally suited for blood samples and other cells in suspension.
-Data can be re-analyzed multiple times after acquisition.
-Flow cytometry files (FCS) can be archived.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What kinds of applications can I run on a flow cytometer?

There are several applications, some of which include immunophenotyping, cell cycle analysis, apoptosis assays such as annexin V staining, CellEvent Caspase-3/7 assay, and TUNEL assay, cell viability, proliferation assays such as CellTrace assay and Click-iT EdU assay, measurements of mitochondrial potential with MitoProbe assays, and cell counting using counting beads.

Find additional tips, troubleshooting help, and resources within our Flow Cytometry Support Center.

Citations & References (16)

Citations & References

Abstract

Cysteine 203 of cyclophilin D is critical for cyclophilin D activation of the mitochondrial permeability transition pore.

Authors:Nguyen TT, Stevens MV, Kohr M, Steenbergen C, Sack MN, Murphy E,

Journal:J Biol Chem

PubMed ID:21930693

The mitochondrial permeability transition pore (mPTP) opening plays a critical role in mediating cell death during ischemia/reperfusion (I/R) injury. Our previous studies have shown that cysteine 203 of cyclophilin D (CypD), a critical mPTP mediator, undergoes protein S-nitrosylation (SNO). To investigate the role of cysteine 203 in mPTP activation, we ... More

Transient and long-lasting openings of the mitochondrial permeability transition pore can be monitored directly in intact cells by changes in mitochondrial calcein fluorescence.

Authors:Petronilli V, Miotto G, Canton M, Brini M, Colonna R, Bernardi P, Di Lisa F

Journal:Biophys J

PubMed ID:9929477

'The occurrence and the mode of opening of the mitochondrial permeability transition pore (MTP) were investigated directly in intact cells by monitoring the fluorescence of mitochondrial entrapped calcein. When MH1C1 cells and hepatocytes were loaded with calcein AM, calcein was also present within mitochondria, because (i) its mitochondrial signal was ... More

Calpain and caspase orchestrated death signal to accomplish apoptosis induced by resveratrol and its novel analog hydroxystilbene-1 [correction of hydroxstilbene-1] in cancer cells.

Authors:Guha P, Dey A, Dhyani MV, Sen R, Chatterjee M, Chattopadhyay S, Bandyopadhyay SK,

Journal:J Pharmacol Exp Ther

PubMed ID:20484155

'Stomach ulceration is a major side effect of most chemopreventive drugs. We have established that although resveratrol is a promising chemopreventive compound, it delays the ulcer healing process. However, its analog hydroxystilbene-1 (HST-1) was devoid of such an ulcerogenic side effect. Consequently, here we tried to explore the chemopreventive efficacy ... More

Secondary coenzyme Q10 deficiency triggers mitochondria degradation by mitophagy in MELAS fibroblasts.

Authors:Cotán D, Cordero MD, Garrido-Maraver J, Oropesa-Ávila M, Rodríguez-Hernández A, Gómez Izquierdo L, De la Mata M, De Miguel M, Lorite JB, Infante ER, Jackson S, Navas P, Sánchez-Alcázar JA,

Journal:FASEB J

PubMed ID:21551238

Mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS) is a mitochondrial disease most usually caused by point mutations in tRNA genes encoded by mtDNA. Here, we report on how this mutation affects mitochondrial function in primary fibroblast cultures established from 2 patients with MELAS who harbored the A3243G mutation. Both ... More

Imaging the mitochondrial permeability transition pore in intact cells.

Authors:Petronilli V, Miotto G, Canton M, Colonna R, Bernardi P, Di Lisa F

Journal:Biofactors

PubMed ID:9914828

The involvement of mitochondrial permeability transition pore (MTP) in cellular processes is generally investigated by indirect means, such as changes in mitochondrial membrane potential or pharmacological inhibition. However, such effects could not be related univocally to MTP. In addition, source of errors could be represented by the increased retention of ... More

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