NeuroTrace™ 500/525 Green Fluorescent Nissl Stain - Solution in DMSO

Citas y referencias (22)

Invitrogen™

NeuroTrace™ 500/525 Green Fluorescent Nissl Stain - Solution in DMSO

La tinción Nissl fluorescente verde NeuroTrace, disponible solo en Molecular Probes, se une a la sustancia Nissl, que está presenteMás información

Número de catálogo	Cantidad
N21480	1 mL

Número de catálogo N21480

Precio (MXN)

Cantidad:

La tinción Nissl fluorescente verde NeuroTrace, disponible solo en Molecular Probes, se une a la sustancia Nissl, que está presente exclusivamente en el soma de las células neuronales. La tinción Nissl NeuroTrace muestra una fluorescencia verde brillante que es visible con filtros apropiados para la fluoresceína y es muchas veces más sensible que la tinción cresilo violeta. La tinción Nissl fluorescente verde NeuroTrace es una mejora del colorante verde FluoroNissl clásico.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorVerde

Método de detecciónFluorescencia

Para utilizar con (equipo)Microscopio de fluorescencia

Tipo de productoTinción de Nissl

Cantidad1 mL

Condiciones de envíoTemperatura ambiente

Localización subcelularCuerpos de Nissl

Excitation/Emission500/525 nm

Línea de productosNEUROTRACE

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.

Preguntas frecuentes

I am labeling brain cryosections with a NeuroTrace Nissl Stain. Is this compatible with antibody labeling?

Yes. We have done this successfully with an anti-GFAP primary and an Alexa Fluor secondary antibody. We would recommend labeling with the primary and secondary antibodies first, then following up with the standard NeuroTrace Nissl Stain protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I can use the NeuroTrace Nissl stains for staining glia or other cell types. What can I do to improve the staining so that it is more selective for neurons?

Our NeuroTrace Nissl stains label the Nissl substance which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells. These dyes are not completely specific for neurons, but will selectively stain neurons based on their high level of protein synthesis. In some cases they may show staining of other cell types such as glia, so you may need to decrease the staining concentration to obtain more selective neuronal labeling. We suggest dilutions in the range of 20- to 300-fold for neuronal staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the NeuroTrace Nissl stains be used on paraffin sections?

We have only tested them on mouse brain cryosections, however, there is at least one citation describing their use on paraffin tissue sections (Michelle L. Schlief, Ann Marie Craig, and Jonathan D. Gitlin. NMDA Receptor Activation Mediates Copper Homeostasis in Hippocampal Neurons. The Journal of Neuroscience, January 5, 2005, 25(1):239 - 246).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the NeuroTrace Nissl stains?

The dyes are proprietary, however they are stains that label the Nissl substance, which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What products do you have for neuronal tracing?

Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (22)

Citations & References

Abstract

Beyond the olfactory bulb: an odotopic map in the forebrain.

Authors:Nikonov AA, Finger TE, Caprio J

Journal:Proc Natl Acad Sci U S A

PubMed ID:16339016

'We report electrophysiological evidence that a simple odotopy, the spatial mapping of different odorants, is maintained above the level of the olfactory bulb (OB). Three classes of biologically relevant odorants for fish are processed in distinct regions of the forebrain (FB) in the channel catfish. Feeding cues, mainly amino acids ... More

JNK-mediated induction of cyclooxygenase 2 is required for neurodegeneration in a mouse model of Parkinson's disease.

Authors:Hunot S, Vila M, Teismann P, Davis RJ, Hirsch EC, Przedborski S, Rakic P, Flavell RA

Journal:Proc Natl Acad Sci U S A

PubMed ID:14704277

'Parkinson''s disease (PD) is a neurodegenerative disorder characterized by loss of dopamine-containing neurons, but the molecular pathways underlying its pathogenesis remain uncertain. Here, we show that by eliminating c-Jun N-terminal kinases (JNKs) we can prevent neurodegeneration and improve motor function in an animal model of PD. First, we found that ... More

Defects in pancreatic development and glucose metabolism in SMN-depleted mice independent of canonical spinal muscular atrophy neuromuscular pathology.

Authors:Bowerman M, Michalski JP, Beauvais A, Murray LM, DeRepentigny Y, Kothary R,

Journal:

PubMed ID:24497575

Spinal muscular atrophy (SMA) is characterized by motor neuron loss, caused by mutations or deletions in the ubiquitously expressed survival motor neuron 1 (SMN1) gene. We recently identified a novel role for Smn protein in glucose metabolism and pancreatic development in both an intermediate SMA mouse model (Smn(2B/-)) and type ... More

Cortical control of adaptation and sensory relay mode in the thalamus.

Authors:Mease RA, Krieger P, Groh A,

Journal:

PubMed ID:24748112

A major synaptic input to the thalamus originates from neurons in cortical layer 6 (L6); however, the function of this cortico-thalamic pathway during sensory processing is not well understood. In the mouse whisker system, we found that optogenetic stimulation of L6 in vivo results in a mixture of hyperpolarization and ... More

High-throughput morphometric analysis of individual neurons.

Authors:Wu CC, Reilly JF, Young WG, Morrison JH, Bloom FE

Journal:Cereb Cortex

PubMed ID:15054070

To facilitate high-throughput quantitative analysis of neuronal structure, this study optimized the diOlistic method of whole neuron labeling to examine multiple neurons in fixed brain, and optimized image acquisition parameters to preserve signal for subsequent photoconversion. Fluorescent dye-coated gold particles were successively delivered by helium-powered ejection to 250 microm thick ... More

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