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Citas y referencias (90)
Invitrogen™
NP-EGTA (o-Nitrophenyl EGTA, Tetrapotassium Salt, cell impermeant
El EGTA enjaulado en NP, sal de potasio es un quelante fotolábil impenetrable en las células que exhibe una altaMás información
| Número de catálogo | Cantidad |
|---|---|
| N6802 también denominado N-6802 | 1 mg |
Número de catálogo N6802
también denominado N-6802
Precio (MXN)
-
Cantidad:
1 mg
El EGTA enjaulado en NP, sal de potasio es un quelante fotolábil impenetrable en las células que exhibe una alta selectividad para Ca2+ en la iluminación UV, su Kd aumenta de 80 nM a > 1 mM. La sal EGTA-NP puede incluir Ca2+ para generar un complejo de calcio enjaulado que rápidamente proporcionará Ca2+ sobre la fotolisis.
Obtenga más información sobre los indicadores de iones, incluidos los indicadores de calcio, potasio, pH y potencial de la membrana ›
Obtenga más información sobre los indicadores de iones, incluidos los indicadores de calcio, potasio, pH y potencial de la membrana ›
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de coloranteQuelante (no fluorescente)
Cantidad1 mg
Condiciones de envíoTemperatura ambiente
Tipo de productoNP-EGTA
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.
Citations & References (90)
Citations & References
Abstract
Spatially organised mitochondrial calcium uptake through a novel pathway in chick neurones.
Journal:Cell Calcium
PubMed ID:16338004
'A brief depolarisation of chick sensory neurones evokes a calcium increase in mitochondria that peaks 1-2s after the depolarisation event and then decays over tens of seconds. Peripheral mitochondria take up more calcium than do central ones, even when the cytosolic calcium increase is spatially homogeneous. The calcium influx into
Ion selectivities of the Ca(2+) sensors for exocytosis in rat phaeochromocytoma cells.
Journal:J Physiol
PubMed ID:11410622
'1. The ion selectivities of the Ca(2+) sensors for the two components of exocytosis in rat phaeochromocytoma (PC12) cells were examined by measurement of membrane capacitance and amperometry. The cytosolic concentrations of metal ions were increased by photolysis of caged-Ca(2+) compounds and measured with low-affinity indicators benzothiazole coumarin (BTC) or
Early requirement for alpha-SNAP and NSF in the secretory cascade in chromaffin cells.
Journal:EMBO J
PubMed ID:10369670
'NSF and alpha-SNAP have been shown to be required for SNARE complex disassembly and exocytosis. However, the exact requirement for NSF and alpha-SNAP in vesicular traffic through the secretory pathway remains controversial. We performed a study on the kinetics of exocytosis from bovine chromaffin cells using high time resolution capacitance
Roles of Ca2+ and crossbridge kinetics in determining the maximum rates of Ca2+ activation and relaxation in rat and guinea pig skinned trabeculae.
Journal:Circ Res
PubMed ID:9686757
'We examined the influences of Ca2+ and crossbridge kinetics on the maximum rate of force development during Ca2+ activation of cardiac myofibrils and on the maximum rate of relaxation. Flash photolysis of diazo-2 or nitrophenyl-EGTA was used to produce a sudden decrease or increase, respectively, in [Ca2+] within Triton-skinned trabeculae
Ca2+ entry in CHO cells, after Ca2+ stores depletion, is mediated by arachidonic acid.
Journal:Cell Calcium
PubMed ID:9883283
'Transfected Chinese hamster ovary cells expressing the rat neurotensin receptor (CHO-NTR cells) were used to study the 'Ca2+ stores depletion-Ca2+ entry' coupling which follows stimulation with neurotensin and liberation of inositol 1,4,5-trisphosphate. This coupling could be dissociated in time: the stores were emptied by stimulation with neurotensin in the absence