Oregon Green 488 BAPTA-1, sal hexapotásica es un indicador de calcio excitable con luz visible. Esta forma de sal solubleMás información
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Número de catálogo
Cantidad
O6806
500 μg
Número de catálogo O6806
Precio (MXN)
-
Cantidad:
500 μg
Oregon Green 488 BAPTA-1, sal hexapotásica es un indicador de calcio excitable con luz visible. Esta forma de sal soluble en agua es útil para la carga intracelular mediante microinyección, infusión con una pipeta de parche o captación inducida mediante nuestro reactivo de carga celular pinocítica Influx (I-14402). También hay disponible una forma de éster AM impenetrable en las células del indicador (O-6807).
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Tipo de coloranteA base de colorantes fluorescentes
Cantidad500 μg
Condiciones de envíoTemperatura ambiente
Para utilizar con (equipo)Microscopio de fluorescencia, Citómetro de flujo, Lector de microplacas
Línea de productosOregon Green
Tipo de productoIndicador de calcio
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.
Citations & References (92)
Citations & References
Abstract
Intracellular astrocyte calcium waves in situ increase the frequency of spontaneous AMPA receptor currents in CA1 pyramidal neurons.
Authors:Fiacco TA, McCarthy KD
Journal:J Neurosci
PubMed ID:14736858
'Spontaneous neurotransmitter release and activation of group I metabotropic glutamate receptors (mGluRs) each play a role in the plasticity of neuronal synapses. Astrocytes may contribute to short- and long-term synaptic changes by signaling to neurons via these processes. Spontaneous whole-cell AMPA receptor (AMPAR) currents were recorded in CA1 pyramidal cells ... More
Formation and function of synapses with respect to Schwann cells at the end of motor nerve terminal branches on mature amphibian (Bufo marinus) muscle.
Authors:Macleod GT, Dickens PA, Bennett MR
Journal:J Neurosci
PubMed ID:11264312
'A study has been made of the formation and regression of synapses with respect to Schwann cells at the ends of motor nerve terminal branches in mature toad (Bufo marinus) muscle. Synapse formation and regression, as inferred from the appearance and loss of N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino)styryl) pyridinium dibromide (FM1-43)-stained vesicle clusters, occurred ... More
Ca2+ fluorescence imaging with pico- and femtosecond two-photon excitation: signal and photodamage.
Authors:Koester HJ, Baur D, Uhl R, Hell SW
Journal:Biophys J
PubMed ID:10512842
'The signal and limitations of calcium florescence imaging using nonresonant multiphoton absorption of near-infrared femto- and picosecond laser pulses were examined. The fluorescence changes of various Ca(2+)-indicators induced by transient increases of the intradendritic calcium concentration were evaluated by evoking physiological activity in neocortical neurons in rat brain slices. Photodamage ... More
Action potentials reliably invade axonal arbors of rat neocortical neurons.
Authors:Cox CL, Denk W, Tank DW, Svoboda K
Journal:Proc Natl Acad Sci U S A
PubMed ID:10931955
'Neocortical pyramidal neurons have extensive axonal arborizations that make thousands of synapses. Action potentials can invade these arbors and cause calcium influx that is required for neurotransmitter release and excitation of postsynaptic targets. Thus, the regulation of action potential invasion in axonal branches might shape the spread of excitation in ... More
Buffer kinetics shape the spatiotemporal patterns of IP3-evoked Ca2+ signals.
Authors:Dargan SL, Parker I
Journal:J Physiol
PubMed ID:14555715
'Ca2+ liberation through inositol 1,4,5-trisphosphate receptors (IP3Rs) plays a universal role in cell regulation, and specificity of cell signalling is achieved through the spatiotemporal patterning of Ca2+ signals. IP3Rs display Ca2+-induced Ca2+ release (CICR), but are grouped in clusters so that regenerative Ca2+ signals may remain localized to individual clusters, ... More