PowerLoad™ Concentrate, 100X

PowerLoad™ es una formulación optimizada de polioles surfactantes no iónicos Pluronic™ para la solubilización de tintes no solubles en aguaMás información

Número de catálogo	Cantidad
P10020	5 mL

Número de catálogo P10020

Precio (MXN)

Cantidad:

PowerLoad™ es una formulación optimizada de polioles surfactantes no iónicos Pluronic™ para la solubilización de tintes no solubles en agua y otros materiales en medios fisiológicos. Estos surfactantes, por ejemplo, el Pluronic™ F-127, se han utilizado para ayudar a dispersar los ésteres de acetoximetil (AM) de los indicadores de iones fluorescentes como fluo-4, fura-2, indo-1, fluo-3 y SBFI; parecen ser necesarios para la carga de otros tintes (por ejemplo, SBFI-AM O PBFI-AM). El uso de PowerLoad™ es opcional con indicadores rojos de calcio desplazado y otros colorantes de éster AM de peso molecular grande. También puede ser útil para la dispersión de otras sondas lipofílicas. La concentración de surfactantes Pluronic™ en PowerLoad™ es inferior al 0,2 %. PowerLoad™ es eficaz si se combina con Probenecid (P36400) soluble en agua para ayudar a la carga de tinte de éster AM y a la retención en las células que extruyen activamente la forma desacetilada a través de bombas de anión. Juntos, estos reactivos permiten una carga máxima de tintes con un mínimo de esfuerzo tanto en aplicaciones de imágenes como de cribado de alto rendimiento (HTS). Se deben realizar controles pertinentes para asegurarse de que PowerLoad™ no está alterando las propiedades de la membrana de la célula.

Obtenga más información sobre los indicadores de iones, incluidos los indicadores de calcio, potasio, pH y potencial de membrana ›

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Almacenamiento recomendadoAlmacenar en el refrigerador (de 2–8 °C).

Cantidad5 mL

Unit SizeEach

Preguntas frecuentes

Can the Fluo-4 Calcium Imaging Kit (Cat. No. F10489) components be purchased separately?

Yes. The following components are sold separately:
Component B: PowerLoad Concentrate (Cat. No. P10020)
Component C: BackDrop Background Suppressor for Microplate Assays (Cat. No. B10512)
Component D: Probenecid, Water Soluble (Cat. No. P36400)

Citations & References (17)

Citations & References

Abstract

The Ste20 kinases Ste20-related proline-alanine-rich kinase and oxidative-stress response 1 regulate NKCC1 function in sensory neurons.

Authors:Geng Y, Hoke A, Delpire E,

Journal:J Biol Chem

PubMed ID:19307180

'NKCC1 is highly expressed in dorsal root ganglion neurons, where it is involved in gating sensory information. In a recent study, it was shown that peripheral nerve injury results in increased NKCC1 activity, not due to an increase in cotransporter expression, but to increased phosphorylation of the cotransporter (Pieraut, S., ... More

A novel initiation mechanism of death in Streptococcus pneumoniae induced by the human milk protein-lipid complex HAMLET and activated during physiological death.

Authors:Clementi EA, Marks LR, Duffey ME, Hakansson AP,

Journal:J Biol Chem

PubMed ID:22700972

'To cause colonization or infection, most bacteria grow in biofilms where differentiation and death of subpopulations is critical for optimal survival of the whole population. However, little is known about initiation of bacterial death under physiological conditions. Membrane depolarization has been suggested, but never shown to be involved, due to ... More

Osteoclast response to low extracellular sodium and the mechanism of hyponatremia-induced bone loss.

Authors:Barsony J, Sugimura Y, Verbalis JG,

Journal:J Biol Chem

PubMed ID:21135109

'Our recent animal and human studies revealed that chronic hyponatremia is a previously unrecognized cause of osteoporosis that is associated with increased osteoclast numbers in a rat model of the human disease of the syndrome of inappropriate antidiuretic hormone secretion (SIADH). We used cellular and molecular approaches to demonstrate that ... More

Receptor-selective diffusion barrier enhances sensitivity of astrocytic processes to metabotropic glutamate receptor stimulation.

Authors:Arizono M, Bannai H, Nakamura K, Niwa F, Enomoto M, Matsu-Ura T, Miyamoto A, Sherwood MW, Nakamura T, Mikoshiba K,

Journal:Sci Signal

PubMed ID:22472649

Metabotropic glutamate receptor (mGluR)-dependent calcium ion (Ca²+) signaling in astrocytic processes regulates synaptic transmission and local blood flow essential for brain function. However, because of difficulties in imaging astrocytic processes, the subcellular spatial organization of mGluR-dependent Ca²+ signaling is not well characterized and its regulatory mechanism remains unclear. Using genetically ... More

Transient Ca2+ mobilization caused by osmotic shock initiates salmonid fish sperm motility.

Authors:Takei GL, Mukai C, Okuno M,

Journal:J Exp Biol

PubMed ID:22279070

Salmonid fish sperm motility is known to be suppressed in millimolar concentrations of extracellular K(+), and dilution of K(+) upon spawning triggers cAMP-dependent signaling for motility initiation. In a previous study, however, we demonstrated that suspending sperm in a 10% glycerol solution and subsequent dilution into a low-osmotic solution induced ... More

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