Yoduro de propidio - solución de 1,0 mg/ml en agua
Yoduro de propidio - solución de 1,0 mg/ml en agua
Citas y referencias (1088)
Invitrogen™

Yoduro de propidio - solución de 1,0 mg/ml en agua

El yoduro de propidio (PI) es una contratinción popular cromosómica o nuclear fluorescente rojo. Dado que el yoduro de propidioMás información
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Número de catálogoCantidad
P356610 mL
Número de catálogo P3566
Precio (MXN)
-
Cantidad:
10 mL
El yoduro de propidio (PI) es una contratinción popular cromosómica o nuclear fluorescente rojo. Dado que el yoduro de propidio no penetra en las células vivas, también se suele utilizar para detectar células muertas en una población.

El PI se une al ADN mediante una intercalación entre las bases con poca o ninguna preferencia de secuencia. En solución acuosa, el colorante posee una excitación/emisión de máxima de 493/636 nm. Una vez que el colorante se ha unido, su fluorescencia mejora de 20 a 30 veces, el máximo de excitación de fluorescencia se desplaza ∼30–40 nm hacia el rojo y el máximo de emisión de fluorescencia se desplaza ∼15 nm al azul, dando como resultado una excitación máxima a 535 nm y una emisión de fluorescencia máxima a 617 nm.

El PI se utiliza ampliamente en microscopía de fluorescencia, microscopía de barrido láser confocal, citometría de flujo y fluorometría.

Obtenga más información sobre el yoduro de propidio y los productos que contienen yoduro de propidio
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Tipo de coloranteYoduro de propidio
FormularioSolución
Cantidad10 mL
Condiciones de envíoTemperatura ambiente
Localización subcelularCitoplasma & citosol
Emission533⁄617
Para utilizar con (aplicación)Ensayo de viabilidad
Para utilizar con (equipo)Microscopio de fluorescencia, Citómetro de flujo
Tipo de productoYoduro de propidio
Unit SizeEach
Contenido y almacenamiento
Contiene 1 frasco de yoduro de propidio (solución de 1,0 mg/ml en agua).
Almacenar en el refrigerador (2–8 °C) y proteger de la luz.

Preguntas frecuentes

I have a LIVE/DEAD BacLight Bacterial Viability kit that has SYTO 9 and propidium iodide in it. Will I be able to stain eukaryotic cells that have engulfed bacteria and determine if the bacteria are alive or dead using this kit?

Unfortunately, no. SYTO 9 will label the nuclei of live or dead cells, including the eukaryotic cells. Propidium iodide is cell impermeant, and will only enter dead cells. If the eukaryotic cells are dead, they will label with propidium iodide as well. If the eukaryotic cells are alive, propidium iodide will not be able to enter and thus will not label the bacteria inside, whether the bacteria are alive or dead. We are not aware of any way to do a viability assay of bacteria once they have been engulfed by cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is propidium iodide (PI) fixable with glutaraldehyde or paraformaldehyde (PFA)?

PI is not fixable with glutaraldehyde or PFA. Both reagents fix by crosslinking amines. PI and other nucleic acid stains do not inherently bind covalently to nucleic acids and these fixatives do not crosslink the dyes to nucleic acids.
The one fixable nucleic acid stain is Ethidium Monoazide Bromide (EMA), Cat no. E1374); it covalently binds to nucleic acids upon activation by exposure to light.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (1088)

Citations & References
Abstract
Signaling through MHC class II molecules blocks CD95-induced apoptosis.
Authors:Catlett IM,Xie P,Hostager BS,Bishop GA
Journal:Journal of immunology (Baltimore, Md. : 1950)
PubMed ID:11342618
Glutamate and non-glutamate receptor mediated toxicity caused by oxygen and glucose deprivation in organotypic hippocampal cultures.
Authors:Newell DW, Barth A, Papermaster V, Malouf AT
Journal:J Neurosci
PubMed ID:7472521
In vitro ischemia models have utilized oxygen, or oxygen and glucose deprivation to simulate ischemic neuronal injury. Combined oxygen and glucose deprivation can induce neuronal damage which is in part mediated through NMDA receptors. Severe oxygen deprivation alone however can cause neuronal injury which is not NMDA mediated. We tested ... More
Identification and characterization of two subpopulations of Encephalitozoon intestinalis.
Authors:Hoffman RM, Marshall MM, Polchert DM, Jost BH
Journal:Appl Environ Microbiol
PubMed ID:12902292
Microsporidia are obligate intracellular protozoa that have been shown to be pathogenic to most living creatures. The development of in vitro cell culture propagation methods has provided researchers with large numbers of spores and facilitated the study of these organisms. Here, we describe heterogeneity within cell culture-propagated Encephalitozoon intestinalis suspensions. ... More
Autoantigens targeted in systemic lupus erythematosus are clustered in two populations of surface structures on apoptotic keratinocytes.
Authors:Casciola-Rosen LA, Anhalt G, Rosen A
Journal:J Exp Med
PubMed ID:7511686
'Systemic lupus erythematosus is a multisystem autoimmune disease in which the autoantibody response targets a variety of autoantigens of diverse subcellular location. We show here that these autoantigens are clustered in two distinct populations of blebs at the surface of apoptotic cells. The population of smaller blebs contains fragmented endoplasmic ... More
Caspase activation contributes to delayed death of heat-stressed striatal neurons.
Authors:White MG, Emery M, Nonner D, Barrett JN
Journal:J Neurochem
PubMed ID:14622126
'Hyperthermia can contribute to brain damage both during development and post-natally. We used rat embryonic striatal neurons in culture to study mechanisms underlying hyperthermia-induced neuronal death. Heat stress at 43 degrees C for 2 h produced no obvious signs of damage during the first 12 h after the stress, but ... More
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