Reactivo Hoechst 33342 Ready Flow™
Reactivo Hoechst 33342 Ready Flow™
Citas y referencias (6)
Invitrogen™

Reactivo Hoechst 33342 Ready Flow™

El reactivo Hoechst 33342 Ready Flow™ es un colorante de color brillante y fácil de usar para el análisis delMás información
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Número de catálogoCantidad
R37165120 ensayos
Número de catálogo R37165
Precio (MXN)
-
Cantidad:
120 ensayos
El reactivo Hoechst 33342 Ready Flow™ es un colorante de color brillante y fácil de usar para el análisis del ciclo celular en un citómetro de flujo. Este tinte emite fluorescencia azul cuando se une a ADN bicatenario con una emisión máxima a 461 nm.

• Estable a temperatura ambiente
• Formato práctico, listo para usar: sin necesidad de diluir, pesar ni pipetear
• Permeable a las células y eficaz en la tinción de células fijas y vivas para el ciclo celular

El reactivo Hoechst 33342 Ready Flow es una tinción de ADN fluorescente azul permeable a las células para el análisis del ciclo celular. Añada 2 gotas del cuentagotas directamente a 1 x 106 células, incube y analice.

El reactivo Hoechst 33342 se une preferentemente a las regiones adenina-timina de ADN bicatenario. Este tinte se une a los surcos menores del ADN y muestra espectros de emisión de fluorescencia que dependen de la relación de pares tinte:base. El reactivo Hoechst 33342 Ready Flow se excita con la luz UV y tiene una emisión máxima a 460 nm.

Especificaciones
ColorAzul
Tipo de colorantePermeabilidad celular
Intervalo de longitud de onda de excitación361/497
Línea de productosReady Flow
Cantidad120 ensayos
Tipo de productoReactivo
SubCellular LocalizationNucleus
Unit SizeEach
Contenido y almacenamiento
Dos frascos cuentagotas, almacenar a temperatura ambiente.

Preguntas frecuentes

Is DAPI a good live-cell nuclear label?

DAPI is considered a semi-permeant/impermeant nucleic acid stain. Staining of nucleic is dependent upon the cell line in its performance. Some cell lines will label with DAPI, others not at all, and others label inconsistenly. Instead, we recommend using either Hoechst 33342 or Hoechst 33258, which have the same wavelength and binding mode as DAPI (at the A-T minor groove) but are readily cell-permeant.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label the nuclei of live cells and track them over time. Can I use DAPI for this?

We do not recommend doing this. DAPI is considered to be a semi-permeant/impermeant nucleic acid stain. DAPI staining of live cells may be inconsistent. It is best used as a counterstain for fixed samples. Other cell permeable nucleic acid stains, such as Hoechst or the SYTO dyes may affect cellular function.

For mammalian cells, we recommend using the CellLight Nucleus transduction reagents, available in CFP, GFP and RFP. With these reagents, the cells are transduced overnight in a single labeling step and the next day the nuclei will fluoresce. The label may be retained for 3-5 days and should not affect cell function. Cytoplasmic cell tracking dyes such as the CellTracker dyes may also be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the concentration of dye/reagent in the Ready Flow products?

The amount of dye or reagent in the Ready Flow products is proprietary. If you need to consider the exact amount of dye or reagent for your experiment, each of the Ready Flow reagents is available as a standalone product.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (6)

Citations & References
Abstract
Sphingosine-1-phosphate mediates the therapeutic effects of bone marrow mesenchymal stem cell-derived microvesicles on articular cartilage defect.
Authors:Xiang C, Yang K, Liang Z, Wan Y, Cheng Y, Ma D, Zhang H, Hou W, Fu P
Journal:Transl Res
PubMed ID:29324234
'Microvesicles (MVs) are emerging as a new mechanism of intercellular communication by transferring cellular components to target cells, yet their function in disease is just being explored. However, the therapeutic effects of MVs in cartilage injury and degeneration remain unknown. We found MVs contained high levels of sphingosine-1-phosphate (S1P) compared ... More
The Importance of Multifrequency Impedance Sensing of Endothelial Barrier Formation Using ECIS Technology for the Generation of a Strong and Durable Paracellular Barrier.
Authors:Robilliard LD, Kho DT, Johnson RH, Anchan A, O'Carroll SJ, Graham ES
Journal:Biosensors (Basel)
PubMed ID:29973526
'In this paper, we demonstrate the application of electrical cell-substrate impedance sensing (ECIS) technology for measuring differences in the formation of a strong and durable endothelial barrier model. In addition, we highlight the capacity of ECIS technology to model the parameters of the physical barrier associated with (I) the paracellular ... More
Diverse Signaling by TGFß Isoforms in Response to Focal Injury is Associated with Either Retinal Regeneration or Reactive Gliosis.
Authors:Conedera FM, Quintela Pousa AM, Presby DM, Mercader N, Enzmann V, Tschopp M
Journal:Cell Mol Neurobiol
PubMed ID:32219603
'Müller cells may have stem cell-like capability as they regenerate photoreceptor loss upon injury in some vertebrates, but not in mammals. Indeed, mammalian Müller cells undergo major cellular and molecular changes summarized as reactive gliosis. Transforming growth factor beta (TGFß) isoforms are multifunctional cytokines that play a central role, both ... More
ECIS technology reveals that monocytes isolated by CD14+ve selection mediate greater loss of BBB integrity than untouched monocytes, which occurs to a greater extent with IL-1ß activated endothelium in comparison to TNFa.
Authors:Kho DT, Johnson R, Robilliard L, du Mez E, McIntosh J, O'Carroll SJ, Angel CE, Graham ES
Journal:PLoS One
PubMed ID:28732059
We have previously shown that TNFa and IL-1ß differentially regulate the inflammatory phenotype of human brain endothelial cells (hCMVECs). In this regard, IL-1ß treatment was considerably more potent than TNFa at increasing expression of inflammatory chemokines and leukocyte adhesion molecules. We therefore hypothesised that interaction of the hCMVECs with human ... More
Colloid chemistry pitfall for flow cytometric enumeration of viruses in water.
Authors:Dlusskaya EA, Atrazhev AM, Ashbolt NJ
Journal:Water Res X
PubMed ID:31194069
Flow cytomtery (FCM) has become a standard approach to enumerate viruses in water research. However, the nature of the fluorescent signal in flow cytometric analysis of water samples and the mechanism of its formation, have not been addressed for bacteriophages expected in wastewaters. Here we assess the behaviour of fluorescent ... More
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