Tarjetas iBind™

Name: Tarjetas iBind™
SKU: SLF1010X4

Las tarjetas iBind se utilizan con el dispositivo iBind Western, que es un dispositivo de procesamiento de Western automatizado queMás información

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Número de catálogo	Cantidad
SLF1010X4	40 tarjetas
SLF1010	10 tarjetas

2 opciones

Número de catálogo SLF1010X4

Precio (MXN)

Cantidad:

40 tarjetas

Las tarjetas iBind se utilizan con el dispositivo iBind Western, que es un dispositivo de procesamiento de Western automatizado que realiza todos los pasos del flujo de trabajo de Western, desde el bloqueo y el lavado hasta la incubación de anticuerpos. El dispositivo iBind Western realiza los pasos a través del flujo lateral secuencial (SLF). El SLF permite la liberación y el flujo de las soluciones y los anticuerpos a la membrana en el momento adecuado, sin necesidad de una fuente de alimentación externa, agitadores o bandejas.

Obtenga más información sobre los sistemas iBind Western >

Las características de la tarjeta iBind incluyen:
• Matriz de fibra de vidrio permite un flujo óptimo y homogéneo de soluciones de inmunodetección.
• Larga vida útil: permite almacenar las tarjetas iBind durante 12 meses a temperatura ambiente.
• Mejor interacción entre antígenos y anticuerpos: las soluciones fluyen entre la tarjeta iBind y la membrana, lo que maximiza la interacción entre proteínas y anticuerpos.

Las tarjetas iBind, junto con el dispositivo iBind Western y el kit de solución iBind o el kit de solución de detección fluorescente iBind (FD), aportan automatización y simplicidad a su protocolo Western. Cada tarjeta iBind admite una minimembrana, y cada caja contiene 10 tarjetas. Para usar las tarjetas es necesario contar con un dispositivo iBind Western.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Cantidad40 tarjetas

Condiciones de envíoTemperatura ambiente

Capacidad1 minitransferencia por tarjeta

Método de detecciónCromogénico, quimioluminiscente, fluorescente

Para utilizar con (aplicación)Inmunotransferencia (Western Blotting)

Para utilizar con (equipo)Dispositivo iBind Western

Membrane CompatibilityNitrocelulosa, PVDF

Línea de productosiBind

Capacidad de procesamiento1 minitransferencia por tarjeta

TipoTarjeta

Unit Size40 cards

Contenido y almacenamiento

Almacenar a temperatura ambiente.

Preguntas frecuentes

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is there any difference between the iBind Flex Card and the original iBind Card?

Besides being ~1.7 times as wide, the iBind Flex Card also includes alignment lines printed on the back that appear when solution is added to the card to help in proper horizontal placement of multiple mini or vertically cut strip blots.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

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