ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit
ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit
Citas y referencias (8)
Invitrogen™

ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit

Achieve unparalleled precision and sensitivity in your fluorescence-based applications from FISH to real-time PCR. With a streamlined, non-enzymatic labeling process and a broad selection of vibrant dyes, ULYSIS kits ensure your nucleic acids are ready for any challenge.
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Número de catálogoEtiqueta o tinteExcitación/emisión
U21650Alexa Fluor™ 488492/520 nm
U21652Alexa Fluor™ 546555/570 nm
U21654Alexa Fluor™ 594588/615 nm
U21660Alexa Fluor™ 647650/670 nm
Número de catálogo U21650
Precio (MXN)
-
Etiqueta o tinte:
Alexa Fluor™ 488
Excitación/emisión:
492/520 nm

ULYSIS nucleic acid labeling kits provide a unique method for attaching a fluorescent dye to nucleic acids. The labeling reagent in the kit reacts with the N7 of guanine to form a stable coordination complex, and the reaction is simple and fast - just heat denature DNA (5 minutes), add the label (react for 15 minutes), then purify.

Features include:

  • Labeling reaction complete in as little as 15 minutes
  • Available in several Alexa Fluor dye colors
  • Non-enzymatic labeling—avoids the potential biases and limitations associated with enzymatic labeling methods
  • Flexibility—compatible with a wide range of experimental conditions and protocols
  • High sensitivity—provides strong fluorescence signals, enabling detection of low-abundance targets

Reliable labeling with the Universal Linkage System

This series of ULYSIS kits was developed to enable rapid and simple coupling of Alexa Fluor dyes to purine bases in nucleic acid polymers. The method, the Universal Linkage System (ULS), makes use of a platinum dye complex that forms a stable adduct with the N7 position of guanine and, to a lesser extent, adenine bases in DNA, RNA, PNA, and oligonucleotides. The result is a reliable nonenzymatic method for nucleic acid labeling.

Labeling is fast and easy

The labeling reaction typically takes only 15 minutes, and separation of the labeled nucleic acids from the unreacted ULS complex can be accomplished with a simple spin-column procedure. DNA longer than ∼1,000 base pairs require a 10-minute DNase digestion before labeling, which both optimizes labeling and fragments the probe for efficient hybridization.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
ColorVerde
Excitación/emisión492/520 nm
Para utilizar con (aplicación)Dot blot, Northern blot, Southern blot, RNA in situ hybridization, DNA in situ hybridization, Multicolor fluorescence in situ hybridization (mFISH), Comparative genome hybridization (CGH), Microarray analysis
Incluye etiqueta o tinteSí
Método de etiquetadoEtiquetado directo
Línea de productosAlexa Fluor, Ulysis
Tipo de productoKits de etiquetado de ácidos nucleicos
Cantidad1 Kit
Condiciones de envíoTemperatura ambiente
Método de detecciónFluorescencia
Tipo de producto finalSondas (ARN etiquetado), sondas (ADN etiquetado), oligos (etiquetados)
FormatoKit
Labeling TargetADN (general), oligos, ARN (general)
Etiqueta o tinteAlexa Fluor™ 488
Tipo de muestraDNA/RNA
Unit Size1 kit
Contenido y almacenamiento
Almacenar en el congelador (- 5 a - 30 °C) y proteger de la luz.

Necesario pero no incluido: columnas de centrifugación basadas en filtración de gel para la purificación del ADN etiquetado a partir de exceso de reactivo de etiquetado

Preguntas frecuentes

Is ULYSIS labeling compatible with microarray analysis?

Yes, there are numerous examples of ULYSIS labeled probes that have been used in microarray analysis. Here are a few publications for your reference:

- Babak T, Zhang W, Marros Q et al. (2004) Probing microRNAs with microarrays: tissue specificity and functional inference. RNA 10(11):1813-1819.
- Hiley SL, Jackman J, Babak T et al. (2005) Detection and discovery of RNA modifications using microarrays. Nucleic Acids Res 33(1):e2.
- Torchet C, Badis G, Devaux F et al. (2005) The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11(6):928-938.

Can probes labeled with the ULYSIS Nucleic Acid Labeling Kits be stored for later use?

Long-term storage for the ULYSIS labeled probes can be done in just about any kind of buffer, TE, formamide, hybridization buffer, or ethanol. We suggest using your normal storage conditions as long as you protect the probes from light. ULYSIS conjugates are very stable. Avoid phenol.

Do you have any tips on using the ULYSIS Nucleic Acid Labeling Kits for RNA labeling?

A preliminary protocol modifies our DNA-labeling protocol: Do not nuclease-treat the RNA, but label it directly by incubating for 10 minutes at 90°C or 15 minutes at 85°C. Add 2 µg of glycogen for every 1 µg of RNA and purify by ethanol precipitation. Refer to these publications:

- Babak T, Zhang W, Marros Q et al. (2004) Probing microRNAs with microarrays: tissue specificity and functional inference. RNA 10(11):1813-1819.
- Hiley SL, Jackman J, Babak T et al. (2005) Detection and discovery of RNA modifications using microarrays. Nucleic Acids Res 33(1):e2.
- Torchet C, Badis G, Devaux F et al. (2005) The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11(6):928-938.

Can the ULYSIS kits be used on probes longer than 1,000 base pairs or even plasmids?

It might be possible to label larger probes with the ULYSIS Nucleic Acid Labeling Kits, but the dye will likely need to be diluted to avoid (or at least reduce) problems with aggregation. Refer to this publication: Coelho-Castelo AA, Santos Junior RR, Bonato VL et al. (2003) B-lymphocytes in bone marrow or lymph nodes can take up plasmid DNA after intramuscular delivery. Hum Gene Ther 14(13):1279-1285.

How stable is the ULYSIS labeled DNA to high temperature?

An oligonucleotide labeled with a ULYSIS Nucleic Acid Labeling Kit should survive 100°C for 5 minutes, and storage at 68°C overnight should also not cause any dissociation of the complex.

View all ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit FAQs

Citations & References (8)

Citations & References
Abstract
Chromosome and replisome dynamics in E. coli: loss of sister cohesion triggers global chromosome movement and mediates chromosome segregation.
Authors:Bates D, Kleckner N
Journal:Cell
PubMed ID:15960977
'Chromosome and replisome dynamics were examined in synchronized E. coli cells undergoing a eukaryotic-like cell cycle. Sister chromosomes remain tightly colocalized for much of S phase and then separate, in a single coordinate transition. Origin and terminus regions behave differently, as functionally independent domains. During separation, sister loci move far ... More
Characterizing peptide-mediated DNA internalization in human cancer cells.
Authors:Wittrup A, Belting M,
Journal:Methods Mol Biol
PubMed ID:19085116
'Cell penetrating peptides (CPPs) are currently used to deliver various macromolecular cargos to intracellular sites of action both in vitro and in vivo on an experimental basis. During the last few years, even more evidence has accumulated indicating that the main route of entry for most CPPs is through endocytosis ... More
A systemic small RNA signaling system in plants.
Authors:Yoo BC, Kragler F, Varkonyi-Gasic E, Haywood V, Archer-Evans S, Lee YM, Lough TJ, Lucas WJ
Journal:Plant Cell
PubMed ID:15258266
'Systemic translocation of RNA exerts non-cell-autonomous control over plant development and defense. Long-distance delivery of mRNA has been proven, but transport of small interfering RNA and microRNA remains to be demonstrated. Analyses performed on phloem sap collected from a range of plants identified populations of small RNA species. The dynamic ... More
Suspension array analysis of 16S rRNA from Fe- and SO(4)2- reducing bacteria in uranium-contaminated sediments undergoing bioremediation.
Authors:Chandler DP, Jarrell AE, Roden ER, Golova J, Chernov B, Schipma MJ, Peacock AD, Long PE
Journal:Appl Environ Microbiol
PubMed ID:16820459
'A 16S rRNA-targeted tunable bead array was developed and used in a retrospective analysis of metal- and sulfate-reducing bacteria in contaminated subsurface sediments undergoing in situ U(VI) bioremediation. Total RNA was extracted from subsurface sediments and interrogated directly, without a PCR step. Bead array validation studies with total RNA derived ... More
Ebola virus VP35-VP40 interaction is sufficient for packaging 3E-5E minigenome RNA into virus-like particles.
Authors:Johnson RF, McCarthy SE, Godlewski PJ, Harty RN
Journal:J Virol
PubMed ID:16698994
'The packaging of viral genomic RNA into nucleocapsids and subsequently into virions is not completely understood. Phosphoprotein (P) and nucleoprotein (NP) interactions link NP-RNA complexes with P-L (polymerase) complexes to form viral nucleocapsids. The nucleocapsid then interacts with the viral matrix protein, leading to specific packaging of the nucleocapsid into ... More
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