SIA (succinimidyl iodoacetate)
SIA (succinimidyl iodoacetate)
Citations & References (2)
Thermo Scientific™

SIA (succinimidyl iodoacetate)

Thermo Scientific Pierce SIA is a very short (1.5 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetylRead more
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Catalog NumberQuantity
2234950 mg
Catalog number 22349
Price (TWD)
3,090.00
Online offer
Ends: 31-Dec-2025
4,400.00
Save 1,310.00 (30%)
Each
Add to cart
Quantity:
50 mg
Request bulk or custom format
Price (TWD)
3,090.00
Online offer
Ends: 31-Dec-2025
4,400.00
Save 1,310.00 (30%)
Each
Add to cart
Thermo Scientific Pierce SIA is a very short (1.5 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.

Features of SIA:

• Reactive groups: NHS ester and iodoacetyl
• Reactive toward: amino and sulfhydryl groups
• Shortest sulfhydryl-reactive and amine-reactive heterobifunctional crosslinker available; compare to SIAB
• NHS ester reacts with primary amines at pH 7-9 to form a stable amide bond
• Iodoacetyl group reacts with sulfhydryl groups at pH > 7.5 to form stable thioether bond
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO)

Product References:

Crosslinker Application Guide -- search for recent literature references for this product
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityYes
DescriptionSIA
FormSolid
Labeling MethodChemical Labeling
Molecular Weight (g/mol)283.02
PEGylatedNo
Product LinePierce
Quantity50 mg
Reactive MoietyHaloacetyl, NHS Ester
Shipping ConditionAmbient
SolubilityDMF, DMSO
Spacer Arm Length1.5 Å
Water SolubleNo
Chemical ReactivityAmine-Sulfhydryl
CleavableNo
Crosslinker TypeHeterobifunctional
FormatStandard
Product TypeCrosslinker
SpacerShort (<10 Å)
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C protected from moisture.

Frequently asked questions (FAQs)

Can you provide the shelf-life for SIA (succinimidyl iodoacetate)?

SIA (succinimidyl iodoacetate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (2)

Citations & References
Abstract
A central role for the T1 domain in voltage-gated potassium channel formation and function.
Authors:Strang C, Cushman SJ, DeRubeis D, Peterson D, Pfaffinger PJ
Journal:J Biol Chem
PubMed ID:11312262
To interpret the recent atomic structures of the Kv (voltage-dependent potassium) channel T1 domain in a functional context, we must understand both how the T1 domain is integrated into the full-length functional channel protein and what functional roles the T1 domain governs. The T1 domain clearly plays a role in ... More
Formation of protein tyrosine ortho-semiquinone radical and nitrotyrosine from cytochrome c-derived tyrosyl radical.
Authors:Chen YR, Chen CL, Chen W, Zweier JL, Augusto O, Radi R, Mason RP
Journal:J Biol Chem
PubMed ID:14761966
Oxidative alteration of mitochondrial cytochrome c (cyt c) has been linked to disease pathophysiology and is one of the causative factors for pro-apoptotic events. Hydrogen peroxide induces a short-lived cyt c-derived tyrosyl radical as detected by the electron spin resonance (ESR) spin-trapping technique. This investigation was undertaken to characterize the ... More