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Citations & References (94)
Invitrogen™
Di-8-ANEPPS
ANEP dyes are molecules that fluoresce in response to electrical potential changes in their environment. These are fast-response probes thatRead more
| Catalog Number | Quantity |
|---|---|
| D3167 | 5 mg |
Catalog number D3167
Price (TWD)
23,120.00
キャンペーン価格
Ends: 31-Dec-2025
28,900.00Save 5,780.00 (20%)
Each
Quantity:
5 mg
Price (TWD)
23,120.00
キャンペーン価格
Ends: 31-Dec-2025
28,900.00Save 5,780.00 (20%)
Each
ANEP dyes are molecules that fluoresce in response to electrical potential changes in their environment. These are fast-response probes that operate by means of a change in their electronic structure, and consequently their fluorescence properties, in response to a change in the surrounding electric field. Their optical response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells, and intact brains. However, the magnitude of their potential-dependent fluorescence change is often small; fast-response probes typically show a 2-10% fluorescence change per 100 mV. Furthermore, these dyes display a potential-dependent shift in their excitation spectra, thus permitting the quantitation of membrane potential using excitation ratio measurements.
Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›
Potential-Sensitive ANEP Dye Specifications:
• Zwitterionic molecule; is less susceptible to internalization than di-4-ANEPPS, permitting extended observation
• Ex/Em maxima bound to model phospholipid membranes are ∼465/635 nm (but spectral properties are highly dependent on environment)
• Nonfluorescent until bound to membranes
• Soluble in ethanol, DMSO, and DMF (di-2-ANEPEQ is a water-soluble ANEP dye)
• Dye is introduced into cells by direct addition of stock solution to cell culture medium, by using Pluronic™ F-127, or by retrograde labeling
• Fast-response probe, suitable for detecting submillisecond membrane potential changes
Applications for Potentiometric Probes
The plasma membrane of a cell typically has a transmembrane potential of approximately -70 mV (negative inside) as a consequence of K+, Na+, and Cl- concentration gradients that are maintained by active transport processes. Potentiometric probes offer an indirect method of detecting the translocation of these ions.
Increases and decreases in membrane potential-referred to as membrane hyperpolarization and depolarization, respectively-play a central role in many physiological processes, including nerve-impulse propagation, muscle contraction, cell signaling and ion-channel gating. Potentiometric probes are important tools for studying these processes.
Find More ANEP Dyes
We offer ANEP dyes in various forms. Review Fast-Response Probes-Section 22.2 in the Molecular Probes™ Handbook for more information on these probes.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›
Potential-Sensitive ANEP Dye Specifications:
• Zwitterionic molecule; is less susceptible to internalization than di-4-ANEPPS, permitting extended observation
• Ex/Em maxima bound to model phospholipid membranes are ∼465/635 nm (but spectral properties are highly dependent on environment)
• Nonfluorescent until bound to membranes
• Soluble in ethanol, DMSO, and DMF (di-2-ANEPEQ is a water-soluble ANEP dye)
• Dye is introduced into cells by direct addition of stock solution to cell culture medium, by using Pluronic™ F-127, or by retrograde labeling
• Fast-response probe, suitable for detecting submillisecond membrane potential changes
Applications for Potentiometric Probes
The plasma membrane of a cell typically has a transmembrane potential of approximately -70 mV (negative inside) as a consequence of K+, Na+, and Cl- concentration gradients that are maintained by active transport processes. Potentiometric probes offer an indirect method of detecting the translocation of these ions.
Increases and decreases in membrane potential-referred to as membrane hyperpolarization and depolarization, respectively-play a central role in many physiological processes, including nerve-impulse propagation, muscle contraction, cell signaling and ion-channel gating. Potentiometric probes are important tools for studying these processes.
Find More ANEP Dyes
We offer ANEP dyes in various forms. Review Fast-Response Probes-Section 22.2 in the Molecular Probes™ Handbook for more information on these probes.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Quantity5 mg
Shipping ConditionRoom Temperature
Sub Cellular LocalizationCell Membranes & Lipids
ColorOrange
For Use With (Equipment)Fluorescence Microscope
Product TypeANEP Dye
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.
Frequently asked questions (FAQs)
What are the excitation/emission maxima for Di-8-ANEPPS (Cat. No. D3167)?
I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?
What is the difference between fast and slow-response membrane potential probes?
What type of membrane potential indicators do you offer and how should I choose one for my experiment?
Citations & References (94)
Citations & References
Abstract
Journal:
PubMed ID:18198177
Exercise training attenuates coronary smooth muscle phenotypic modulation and nuclear Ca2+ signaling.
Journal:Am J Physiol Heart Circ Physiol
PubMed ID:12388302
'Physical inactivity is an independent risk factor for coronary heart disease, yet the mechanism(s) of exercise-related cardioprotection remains unknown. We tested the hypothesis that coronary smooth muscle after exercise training would have decreased mitogen-induced phenotypic modulation and enhanced regulation of nuclear Ca(2+). Yucatan swine were endurance exercise trained (EX) on
Ratiometric measurement of endothelial depolarization in arterioles with a potential-sensitive dye.
Journal:Am J Physiol
PubMed ID:8764277
'A fluorescence ratio technique based on the voltage-sensitive dye 1-(3-sulfonatopropyl)-8-[beta-[2-di-n-butylamino)-6-naphythyl++ +]vinyl] pyridinium betaine (di-8-ANEPPS)has been developed for recording membrane potential changes during vascular responses of arterioles. Perfusion of hamster cheek pouch arterioles with the dye labeled the endothelial cell layer. voltage responses from the endothelium of intact arterioles were determined
Spatially non-uniform Ca2+ signals induced by the reduction of transverse tubules in citrate-loaded guinea-pig ventricular myocytes in culture.
Journal:J Physiol
PubMed ID:9003546
'1. Ratiometric confocal microscopy and the whole-cell patch clamp technique were used to simultaneously record intracellular Ca2+ transients and membrane currents from guinea-pig ventricular myocytes. Intracellular dialysis with the low-affinity Ca2+ buffer citrate enabled us to record and analyse Ca2+ transients caused by Ca2+ influx alone and by additional Ca2+
The effect of asymmetric surface potentials on the intramembrane electric field measured with voltage-sensitive dyes.
Journal:Biophys J
PubMed ID:12668484
'Ratiometric imaging of styryl potentiometric dyes can be used to measure the potential gradient inside the membrane (intramembrane potential), which is the sum of contributions from transmembrane potential, dipole potential, and the difference in the surface potentials at both sides of the membrane. Here changes in intramembrane potential of the