FluoSpheres™ Carboxylate-Modified Microspheres

Citations & References (9)

Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

Name: FluoSpheres™ Carboxylate-Modified Microspheres
SKU: F8797
Price: 14840.00 TWD

Achieve the brightest fluorescence with Carboxylate-Modified FluoSphere Microspheres, available in different colors and particle sizes.

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Catalog Number	Diameter (Metric)	Color	Excitation/Emission	Quantity
F8797	0.1 μm	Blue	350/440 nm	10 mL
F8803	0.1 μm	Yellow-Green	505/515 nm	10 mL
F8816	1.0 μm	Crimson	625/645 nm	2 mL
F8823	1.0 μm	Yellow-Green	505/515 nm	10 mL
F8801	0.1 μm	Red	580/605 nm	10 mL
F8811	0.2 μm	Yellow-Green	505/515 nm	10 mL
F8807	0.2 μm	Dark Red	660/680 nm	2 mL
F10720	0.04 μm	Yellow-Green, Orange, Red, Dark Red	505/515, 540/560, 580/605, 660/680 nm	1 mL/each
F20881	0.2 μm	Orange	365/610 nm	2 mL
F8783 also known as F-8783	0.02 μm	Dark Red	660/680 nm	2 mL
F8786	0.02 μm	Red	580/605 nm	10 mL
F8795 also known as F-8795	0.04 μm	Yellow-Green	505/515 nm	1 mL
F8813	0.5 μm	Yellow-Green	505/515 nm	10 mL
F8820	1.0 μm	Orange	540/560 nm	10 mL
F8825	2.0 μm	Nile Red	535/575 nm	2 mL
F8827	2.0 μm	Yellow-Green	505/515 nm	2 mL
F8781 also known as F-8781	0.02 μm	Blue	365/415 nm	10 mL
F8782 also known as F-8782	0.02 μm	Crimson	625/645 nm	2 mL
F8784	0.02 μm	Nile Red	535/575 nm	10 mL
F8787	0.02 μm	Yellow-Green	505/515 nm	10 mL
F8789 also known as F-8789	0.04 μm	Dark Red	660/680 nm	1 mL
F8792 also known as F-8792	0.04 μm	Orange	540/560 nm	1 mL
F8793	0.04 μm	Red	580/605 nm	1 mL
F8794	0.04 μm	Red-Orange	565/580 nm	1 mL
F8799	0.1 μm	Infrared	715/755 nm	1 mL
F8800 also known as F-8800	0.1 μm	Orange	540/560 nm	10 mL
F8805	0.2 μm	Blue	365/415 nm	10 mL
F8806	0.2 μm	Crimson	625/645 nm	2 mL
F8809	0.2 μm	Orange	540/560 nm	10 mL
F8810	0.2 μm	Red	580/605 nm	10 mL
F8812	0.5 μm	Red	580/605 nm	10 mL
F8814	1.0 μm	Blue	365/415 nm	10 mL
F8815	1.0 μm	Blue	350/440 nm	10 mL
F8819	1.0 μm	Nile Red	535/575 nm	10 mL
F8821	1.0 μm	Red	580/605 nm	10 mL
F8824 also known as F-8824	2.0 μm	Blue	365/415 nm	2 mL
F8826	2.0 μm	Red	580/605 nm	2 mL

37 Options

Catalog number F8797

Price (TWD)

14,840.00

Online offer

Ends: 31-Dec-2025

21,200.00

Save 6,360.00 (30%)

Each

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Diameter (Metric):

0.1 μm

Color:

Blue

Excitation/Emission:

350/440 nm

Quantity:

10 mL

Price (TWD)

14,840.00

Online offer

Ends: 31-Dec-2025

21,200.00

Save 6,360.00 (30%)

Each

Add to cart

Easily perform flow cytometry, microscopy, HTS, HCS, immunoassay, and other laboratory applications using our extensive selection of FluoSpheres Carboxylate-Modified Microspheres. FluoSphere beads can be used in passive adsorption or active, covalent coupling of proteins, nucleic acids, and biomolecules for particle capture applications. FluoSphere microspheres are loaded with proprietary fluorescent dyes, making them the brightest microspheres available.

Visualize the brightest fluorescence for laboratoy applications including fluorescence microscopy, flow cytometry, HTS, HCS, and cell tracing with our Carboxylate-Modified FluoSphere Microspheres, which are manufactured from polystyrene microspheres and loaded with different proprietary dyes. Using specialized staining methods enables all of the fluorescent dye molecules to be contained inside each polystyrene microsphere instead of on the bead's surface. This protective environment within the bead shields the dye from detrimental environmental effects, such as photobleaching. Our carboxylate-modified microspheres are coated with a hydrophilic polymer containing multiple carboxylic acids for covalent attachment of ligands. A range of particle sizes is available for different research uses and experiments.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation/Emission350/440 nm

Product LineFLUOSPHERES

Quantity10 mL

Surface ModificationCarboxylate

ColorBlue

Diameter (Metric)0.1 μm

For Use With (Application)Fluorescence Microscopy

MaterialPolystyrene

Product TypeCarboxylate-Modified Microsphere

Unit SizeEach

Contents & Storage

Store in refrigerator (2–8°C) and protect from light.

Frequently asked questions (FAQs)

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?

Bacterial contamination is the most common cause of microspheres becoming unusable. Many of our particles are supplied with a low level of sodium azide to prevent bacterial contamination, but sometimes this can still occur. Bacterial contamination is best assessed by plating on appropriate growth medium and checking the plates after 72 hr.

Find additional tips, troubleshooting help, and resources within ourMicrospheres Support Center.

View all FluoSpheres™ Carboxylate-Modified Microspheres FAQs

Citations & References (9)

Citations & References

Abstract

The power of single and multibeam two-photon microscopy for high-resolution and high-speed deep tissue and intravital imaging.

Authors:Niesner R, Andresen V, Neumann J, Spiecker H, Gunzer M,

Journal:Biophys J

PubMed ID:17557785

'Two-photon microscopy is indispensable for deep tissue and intravital imaging. However, current technology based on single-beam point scanning has reached sensitivity and speed limits because higher performance requires higher laser power leading to sample degradation. We utilize a multifocal scanhead splitting a laser beam into a line of 64 foci, ... More

a2 Adrenergic receptor-mediated inhibition of thermogenesis.

Authors:Madden CJ, Tupone D, Cano G, Morrison SF,

Journal:J Neurosci

PubMed ID:23365239

'a2 adrenergic receptor (a2-AR) agonists have been used as antihypertensive agents, in the management of drug withdrawal, and as sedative analgesics. Since a2-AR agonists also influence the regulation of body temperature, we explored their potential as antipyretic agents. This study delineates the central neural substrate for the inhibition of rat ... More

Glucoprivation in the ventrolateral medulla decreases brown adipose tissue sympathetic nerve activity by decreasing the activity of neurons in raphe pallidus.

Authors:Madden CJ,

Journal:Am J Physiol Regul Integr Comp Physiol

PubMed ID:22071154

'In urethane/a-chloralose anesthetized rats, cold exposure increased brown adipose tissue sympathetic nerve activity (BAT SNA: +699 ± 104% control). Intravenous administration of 2-deoxy-D-glucose (2-DG; 200 mg·ml(-1)·kg(-1)) reversed the cold-evoked activation of BAT SNA (nadir: 139 ± 36% of control) and decreased BAT temperature (-1.1 ± 0.2°C), expired CO(2) (-0.4 ± ... More

C/EBPbeta phosphorylation rescues macrophage dysfunction and apoptosis induced by anthrax lethal toxin.

Authors:Buck M, Chojkier M,

Journal:Am J Physiol Cell Physiol

PubMed ID:17855774

Bacillus anthracis lethal toxin (LT) impairs innate and adaptive immunity. Anthrax lethal factor stimulates cleavage of MAPK kinases, which prevents the activation of antiapoptotic MAPK targets. However, these MAPK targets have not been yet identified. Here, we found that LT induces macrophage apoptosis by enhancing caspase 8 activation and by ... More

Central activation of the A1 adenosine receptor (A1AR) induces a hypothermic, torpor-like state in the rat.

Authors:Tupone D, Madden CJ, Morrison SF,

Journal:

PubMed ID:24005302

Since central activation of A1 adenosine receptors (A1ARs) plays an important role in the induction of the hypothermic and hypometabolic torpid state in hibernating mammals, we investigated the potential for the A1AR agonist N6-cyclohexyladenosine to induce a hypothermic, torpor-like state in the (nonhibernating) rat. Core and brown adipose tissue temperatures, ... More

View all FluoSpheres™ Carboxylate-Modified Microspheres citations

FluoSpheres™ Carboxylate-Modified Microspheres

Frequently asked questions (FAQs)

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

I sonicated my 2.0 &micro;m carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

What is the warranty for FluoSpheres microspheres?

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?

Citations & References (9)

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?