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Invitrogen
NGAL Monoclonal Antibody (6B4)
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Product Details
HYB 211-02-02
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
6B4
Immunogen
Human recombinant NGAL in monomer and dimer forms.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Storage conditions
4°C, store in dark
Shipping conditions
Wet ice
RRID
Product Specific Information
HYB 211-02-02 is specific for human NGAL, native and denatured forms. Crossreactivity to other species has not
been determined.
HYB 211-02-02 has been successfully used in Western blotting, ELISA and Immunohistochemistry procedures. HYB 211-02-02 reacts strongly with NGAL. Strong reaction is seen when tested in sandwich ELISA in combination with a polyclonal antibody against the antigen. In immunohistochemical staining of paraformaldehyde fixed
neutrophils HYB 211-02-02 recognizes NGAL. In western blotting HYB 211-02-02 reacts with NGAL in both reduced as
well as unreduced form.
The HYB 211-02-02 immunogen is human recombinant NGAL in monomer and dimer forms adsorbed to aluminum hydroxide gel.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Target Information
NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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