ActinRed 555 ReadyProbes Reagent is a selective, high-affinity F-actin probe (phalloidin) conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). ActinRed 555 reagent is formulated as a room temperature-stable solution of Rhodamine Phalloidin (Cat. No. R415) and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells.

Also available: Rhodamine Phalloidin at 12.5X concentration (65 U/mL) in PBS with BSA and trace amounts of methanol and sodium azide.

• High-affinity staining of F-actin with superior specificity compared to antibody methods
• Outstanding fluorescence performance (Ex/Em: 540/565 nm)
• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
• Stability at room temperature—keep handy at your scope or cell culture area.

See other ReadyProbes reagents for cell staining
Learn more about other stains for actin

Cell imaging applications
Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, ActinRed 555 reagent allows high-contrast discrimination of actin staining.

Suggestions for use
• ActinRed 555 reagent may be added directly to fixed cells in full media or buffer solutions
• In most cases 2 drops/ml and an incubation time of 15 to 30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
• ActinRed 555 dye is excited with a maximum at 540 nm and has an emission maximum at 565 nm. It is detected through standard TRITC (Rhodamine/DiI/Cy3/Alexa Fluor 546) filters. andard TRITC (Rhodamine/DiI/Cy3/Alexa Fluor 546) filters.

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