ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit

Citations & References (8)

Invitrogen™

ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit

Name: ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit
SKU: U21650
Price: 18060.00 TWD

Achieve unparalleled precision and sensitivity in your fluorescence-based applications from FISH to real-time PCR. With a streamlined, non-enzymatic labeling process and a broad selection of vibrant dyes, ULYSIS kits ensure your nucleic acids are ready for any challenge.

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Catalog Number	Label or Dye	Excitation/Emission
U21650	Alexa Fluor 488	492/520 nm
U21660	Alexa Fluor 647	650/670 nm

2 Options

Catalog number U21650

Price (TWD)

18,060.00

Online offer

Ends: 31-Dec-2025

25,800.00

Save 7,740.00 (30%)

1 kit

Add to cart

Label or Dye:

Alexa Fluor 488

Excitation/Emission:

492/520 nm

Price (TWD)

18,060.00

Online offer

Ends: 31-Dec-2025

25,800.00

Save 7,740.00 (30%)

1 kit

Add to cart

ULYSIS nucleic acid labeling kits provide a unique method for attaching a fluorescent dye to nucleic acids. The labeling reagent in the kit reacts with the N7 of guanine to form a stable coordination complex, and the reaction is simple and fast - just heat denature DNA (5 minutes), add the label (react for 15 minutes), then purify.

Features include:

Labeling reaction complete in as little as 15 minutes
Available in several Alexa Fluor dye colors
Non-enzymatic labeling—avoids the potential biases and limitations associated with enzymatic labeling methods
Flexibility—compatible with a wide range of experimental conditions and protocols
High sensitivity—provides strong fluorescence signals, enabling detection of low-abundance targets

Reliable labeling with the Universal Linkage System

This series of ULYSIS kits was developed to enable rapid and simple coupling of Alexa Fluor dyes to purine bases in nucleic acid polymers. The method, the Universal Linkage System (ULS), makes use of a platinum dye complex that forms a stable adduct with the N7 position of guanine and, to a lesser extent, adenine bases in DNA, RNA, PNA, and oligonucleotides. The result is a reliable nonenzymatic method for nucleic acid labeling.

Labeling is fast and easy

The labeling reaction typically takes only 15 minutes, and separation of the labeled nucleic acids from the unreacted ULS complex can be accomplished with a simple spin-column procedure. DNA longer than ∼1,000 base pairs require a 10-minute DNase digestion before labeling, which both optimizes labeling and fragments the probe for efficient hybridization.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

ColorGreen

Excitation/Emission492/520 nm

For Use With (Application)Dot blot, Northern blot, Southern blot, RNA in situ hybridization, DNA in situ hybridization, Multicolor fluorescence in situ hybridization (mFISH), Comparative genome hybridization (CGH), Microarray analysis

Includes Label or DyeYes

Labeling MethodDirect Labeling

Product LineAlexa Fluor, Ulysis

Product TypeNucleic Acid Labeling Kit

Quantity1 Kit

Shipping ConditionRoom Temperature

Detection MethodFluorescence

Final Product TypeProbes (Labeled RNA), Probes (Labeled DNA), Oligos (Labeled)

FormatKit

Labeling TargetDNA (General), Oligos, RNA (General)

Label or DyeAlexa Fluor 488

Sample TypeDNA/RNA

Unit Size1 kit

Contents & Storage

Store in freezer (-5 to -30°C) and protect from light.

Needed but not included: gel filtration-based spin columns for purification of the labeled DNA from excess labeling reagent

Frequently asked questions (FAQs)

Is ULYSIS labeling compatible with microarray analysis?

Yes, there are numerous examples of ULYSIS labeled probes that have been used in microarray analysis. Here are a few publications for your reference:

- Babak T, Zhang W, Marros Q et al. (2004) Probing microRNAs with microarrays: tissue specificity and functional inference. RNA 10(11):1813-1819.
- Hiley SL, Jackman J, Babak T et al. (2005) Detection and discovery of RNA modifications using microarrays. Nucleic Acids Res 33(1):e2.
- Torchet C, Badis G, Devaux F et al. (2005) The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11(6):928-938.

Can probes labeled with the ULYSIS Nucleic Acid Labeling Kits be stored for later use?

Long-term storage for the ULYSIS labeled probes can be done in just about any kind of buffer, TE, formamide, hybridization buffer, or ethanol. We suggest using your normal storage conditions as long as you protect the probes from light. ULYSIS conjugates are very stable. Avoid phenol.

Do you have any tips on using the ULYSIS Nucleic Acid Labeling Kits for RNA labeling?

A preliminary protocol modifies our DNA-labeling protocol: Do not nuclease-treat the RNA, but label it directly by incubating for 10 minutes at 90°C or 15 minutes at 85°C. Add 2 µg of glycogen for every 1 µg of RNA and purify by ethanol precipitation. Refer to these publications:

- Babak T, Zhang W, Marros Q et al. (2004) Probing microRNAs with microarrays: tissue specificity and functional inference. RNA 10(11):1813-1819.
- Hiley SL, Jackman J, Babak T et al. (2005) Detection and discovery of RNA modifications using microarrays. Nucleic Acids Res 33(1):e2.
- Torchet C, Badis G, Devaux F et al. (2005) The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11(6):928-938.

Can the ULYSIS kits be used on probes longer than 1,000 base pairs or even plasmids?

It might be possible to label larger probes with the ULYSIS Nucleic Acid Labeling Kits, but the dye will likely need to be diluted to avoid (or at least reduce) problems with aggregation. Refer to this publication: Coelho-Castelo AA, Santos Junior RR, Bonato VL et al. (2003) B-lymphocytes in bone marrow or lymph nodes can take up plasmid DNA after intramuscular delivery. Hum Gene Ther 14(13):1279-1285.

How stable is the ULYSIS labeled DNA to high temperature?

An oligonucleotide labeled with a ULYSIS Nucleic Acid Labeling Kit should survive 100°C for 5 minutes, and storage at 68°C overnight should also not cause any dissociation of the complex.

View all ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit FAQs

Citations & References (8)

Citations & References

Abstract

Chromosome and replisome dynamics in E. coli: loss of sister cohesion triggers global chromosome movement and mediates chromosome segregation.

Authors:Bates D, Kleckner N

Journal:Cell

PubMed ID:15960977

'Chromosome and replisome dynamics were examined in synchronized E. coli cells undergoing a eukaryotic-like cell cycle. Sister chromosomes remain tightly colocalized for much of S phase and then separate, in a single coordinate transition. Origin and terminus regions behave differently, as functionally independent domains. During separation, sister loci move far ... More

Characterizing peptide-mediated DNA internalization in human cancer cells.

Authors:Wittrup A, Belting M,

Journal:Methods Mol Biol

PubMed ID:19085116

'Cell penetrating peptides (CPPs) are currently used to deliver various macromolecular cargos to intracellular sites of action both in vitro and in vivo on an experimental basis. During the last few years, even more evidence has accumulated indicating that the main route of entry for most CPPs is through endocytosis ... More

A systemic small RNA signaling system in plants.

Authors:Yoo BC, Kragler F, Varkonyi-Gasic E, Haywood V, Archer-Evans S, Lee YM, Lough TJ, Lucas WJ

Journal:Plant Cell

PubMed ID:15258266

'Systemic translocation of RNA exerts non-cell-autonomous control over plant development and defense. Long-distance delivery of mRNA has been proven, but transport of small interfering RNA and microRNA remains to be demonstrated. Analyses performed on phloem sap collected from a range of plants identified populations of small RNA species. The dynamic ... More

Suspension array analysis of 16S rRNA from Fe- and SO(4)2- reducing bacteria in uranium-contaminated sediments undergoing bioremediation.

Authors:Chandler DP, Jarrell AE, Roden ER, Golova J, Chernov B, Schipma MJ, Peacock AD, Long PE

Journal:Appl Environ Microbiol

PubMed ID:16820459

'A 16S rRNA-targeted tunable bead array was developed and used in a retrospective analysis of metal- and sulfate-reducing bacteria in contaminated subsurface sediments undergoing in situ U(VI) bioremediation. Total RNA was extracted from subsurface sediments and interrogated directly, without a PCR step. Bead array validation studies with total RNA derived ... More

Ebola virus VP35-VP40 interaction is sufficient for packaging 3E-5E minigenome RNA into virus-like particles.

Authors:Johnson RF, McCarthy SE, Godlewski PJ, Harty RN

Journal:J Virol

PubMed ID:16698994

'The packaging of viral genomic RNA into nucleocapsids and subsequently into virions is not completely understood. Phosphoprotein (P) and nucleoprotein (NP) interactions link NP-RNA complexes with P-L (polymerase) complexes to form viral nucleocapsids. The nucleocapsid then interacts with the viral matrix protein, leading to specific packaging of the nucleocapsid into ... More

View all ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit citations