FluoSpheres™ Carboxylate-Modified Microspheres
FluoSpheres™ Carboxylate-Modified Microspheres
Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

Achieve the brightest fluorescence with Carboxylate-Modified FluoSphere Microspheres, available in different colors and particle sizes.
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產品號碼Diameter (Metric)ColorExcitation/EmissionQuantity
F8795
亦稱為 F-8795
0.04 μmYellow-Green505/515 nm1 mL
F88030.1 μmYellow-Green505/515 nm10 mL
F88161.0 μmCrimson625/645 nm2 mL
F88231.0 μmYellow-Green505/515 nm10 mL
F88010.1 μmRed580/605 nm10 mL
F88110.2 μmYellow-Green505/515 nm10 mL
F88070.2 μmDark Red660/680 nm2 mL
F107200.04 μmYellow-Green, Orange, Red, Dark Red505/515, 540/560, 580/605, 660/680 nm1 mL/each
F208810.2 μmOrange365/610 nm2 mL
F8783
亦稱為 F-8783
0.02 μmDark Red660/680 nm2 mL
F87860.02 μmRed580/605 nm10 mL
F88130.5 μmYellow-Green505/515 nm10 mL
F88201.0 μmOrange540/560 nm10 mL
F88252.0 μmNile Red535/575 nm2 mL
F88272.0 μmYellow-Green505/515 nm2 mL
F8781
亦稱為 F-8781
0.02 μmBlue365/415 nm10 mL
F8782
亦稱為 F-8782
0.02 μmCrimson625/645 nm2 mL
F87840.02 μmNile Red535/575 nm10 mL
F87870.02 μmYellow-Green505/515 nm10 mL
F8789
亦稱為 F-8789
0.04 μmDark Red660/680 nm1 mL
F8792
亦稱為 F-8792
0.04 μmOrange540/560 nm1 mL
F87930.04 μmRed580/605 nm1 mL
F87940.04 μmRed-Orange565/580 nm1 mL
F87970.1 μmBlue350/440 nm10 mL
F87990.1 μmInfrared715/755 nm1 mL
F8800
亦稱為 F-8800
0.1 μmOrange540/560 nm10 mL
F88050.2 μmBlue365/415 nm10 mL
F88060.2 μmCrimson625/645 nm2 mL
F88090.2 μmOrange540/560 nm10 mL
F88100.2 μmRed580/605 nm10 mL
F88120.5 μmRed580/605 nm10 mL
F88141.0 μmBlue365/415 nm10 mL
F88151.0 μmBlue350/440 nm10 mL
F88191.0 μmNile Red535/575 nm10 mL
F88211.0 μmRed580/605 nm10 mL
F8824
亦稱為 F-8824
2.0 μmBlue365/415 nm2 mL
F88262.0 μmRed580/605 nm2 mL
產品號碼 F8795
亦稱為 F-8795
價格 (TWD)
14,840.00
Online offer
Ends: 31-Dec-2025
21,200.00
您節省 6,360.00 (30%)
Each
新增至購物車
Diameter (Metric):
0.04 μm
Color:
Yellow-Green
Excitation/Emission:
505/515 nm
Quantity:
1 mL
價格 (TWD)
14,840.00
Online offer
Ends: 31-Dec-2025
21,200.00
您節省 6,360.00 (30%)
Each
新增至購物車

Easily perform flow cytometry, microscopy, HTS, HCS, immunoassay, and other laboratory applications using our extensive selection of FluoSpheres Carboxylate-Modified Microspheres. FluoSphere beads can be used in passive adsorption or active, covalent coupling of proteins, nucleic acids, and biomolecules for particle capture applications. FluoSphere microspheres are loaded with proprietary fluorescent dyes, making them the brightest microspheres available.

Visualize the brightest fluorescence for laboratoy applications including fluorescence microscopy, flow cytometry, HTS, HCS, and cell tracing with our Carboxylate-Modified FluoSphere Microspheres, which are manufactured from polystyrene microspheres and loaded with different proprietary dyes. Using specialized staining methods enables all of the fluorescent dye molecules to be contained inside each polystyrene microsphere instead of on the bead's surface. This protective environment within the bead shields the dye from detrimental environmental effects, such as photobleaching. Our carboxylate-modified microspheres are coated with a hydrophilic polymer containing multiple carboxylic acids for covalent attachment of ligands. A range of particle sizes is available for different research uses and experiments.
For Research Use Only. Not for use in diagnostic procedures.
規格
Excitation/Emission505/515 nm
Product LineFLUOSPHERES
Quantity1 mL
Surface ModificationCarboxylate
ColorYellow-Green
Diameter (Metric)0.04 μm
For Use With (Application)Fluorescence Microscopy
MaterialPolystyrene
Product TypeCarboxylate-Modified Microsphere
Unit SizeEach
內容物與存放
Store in refrigerator (2°C to 8°C) and protect from light.

常見問答集 (常見問題)

I read that carboxylate-modified latex (CML) beads have a "fluffy coat" of carboxyl groups on their surface. What is meant by this?

The CML beads have a high density of carboxyl groups at the surface. The surface layer is quite hydrophilic and at the appropriate pH (pH>5), are charged; due to electrostatic repulsion, this type of surface is 3-dimensional and may be considered analogous to the fuzz on a tennis ball.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用資料與參考文獻 (8)

引用資料與參考文獻
Abstract
Nano-sized fluorescent particles as new tracers for sentinel node detection: experimental model for decision of appropriate size and wavelength.
Authors:Nakajima M, Takeda M, Kobayashi M, Suzuki S, Ohuchi N
Journal:Cancer Sci
PubMed ID:15958058
'The concepts of made-to-order and low-invasiveness medicines are becoming widely accepted. A treatment for cancer, with minimum invasive surgery and without lymph nodes dissection based on sentinel lymph node (SN) navigation surgery, would adhere to these concepts. Dyes and/or radioisotopes are employed for SN detection in standard methods, however, each ... More
Why molecules move along a temperature gradient.
Authors:Duhr S, Braun D
Journal:Proc Natl Acad Sci U S A
PubMed ID:17164337
'Molecules drift along temperature gradients, an effect called thermophoresis, the Soret effect, or thermodiffusion. In liquids, its theoretical foundation is the subject of a long-standing debate. By using an all-optical microfluidic fluorescence method, we present experimental results for DNA and polystyrene beads over a large range of particle sizes, salt ... More
Oxidized low-density lipoprotein promotes mature dendritic cell transition from differentiating monocyte.
Authors:Perrin-Cocon L, Coutant F, Agaugué S, Deforges S, André P, Lotteau V
Journal:J Immunol
PubMed ID:11564795
'Proinflammatory oxidized phospholipids are generated during oxidative modification of low-density lipoproteins (LDL). The production of these proinflammatory oxidized phospholipids is controlled by secreted enzymes that circulate as proteins complexed with LDL and high-density lipoprotein. During the acute phase response to tissue injury, profound changes occur in lipoprotein enzymatic composition that ... More
I5S: wide-field light microscopy with 100-nm-scale resolution in three dimensions.
Authors:Shao L, Isaac B, Uzawa S, Agard DA, Sedat JW, Gustafsson MG,
Journal:Biophys J
PubMed ID:18326649
A new type of wide-field fluorescence microscopy is described, which produces 100-nm-scale spatial resolution in all three dimensions, by using structured illumination in a microscope that has two opposing objective lenses. Illumination light is split by a grating and a beam splitter into six mutually coherent beams, three of which ... More
Determination of absolute protein numbers in single synapses by a GFP-based calibration technique.
Authors:Sugiyama Y, Kawabata I, Sobue K, Okabe S,
Journal:Nat Methods
PubMed ID:16118638
To build a quantitative model of molecular organization of neurons, it is essential to have information about the number of protein molecules at individual synapses. Here we developed a method to estimate absolute numbers of individual proteins at actual excitatory synapses by calibrating the fluorescence intensity of microspheres with single ... More