The first aspect of any experiment looking at relative quantitation of gene expression should be the selection of endogenous control gene(s), to normalize for variations in sample input. The TaqMan® Express Human Endogenous Control Plate contains 32 genes ..., plated in triplicate, that have been shown to be good candidates for such an experiment. This collection of genes has been selected from literature searches and/or whole genome microarray tests carried out on numerous human tissues. They have been shown to be expressed constitutively and at moderate abundance across most test samples.
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