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Invitrogen
TOX Monoclonal Antibody (TXRX10), eBioscience™
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FIGURE: 1 / 8
TOX Antibody (14-6502-80) in WB
Western Blot was performed using Anti-TOX Monoclonal Antibody (TXRX10), eBioscience™ (Product # 14-6502-80, 14-6502-82) and 57, 60 kDa bands corresponding to Thymocyte selection-associated high mobility group box protein TOX were observed. Nuclear enriched extracts (40 µg lysate) of Ramos (Lane 1), Daudi (Lane 2), Jurkat (Lane 3), SH-SY5Y (Lane 4), Raji (Lane 5) and tissue extracts (40 µg lysate) of Mouse Heart (Lane 6), Mouse Thymus (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved prot... View More
Product Details
14-6502-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
TXRX10
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This TXRX10 monoclonal antibody reacts with human and mouse TOX, which is also known as Thymocyte selection-associated HMG box protein. This DNA-binding nuclear factor is expressed in thymocytes, T lymphocytes, NK cells, and lymphoid tissue-inducer (LTi) cells. TOX expression in thymocytes is upregulated during beta-selection and positive selection. Studies using Tox knockout mice have demonstrated a critical role for TOX in CD4^+ T cell development as a regulator of the CD4^loCD8^lo to CD4^+CD8^lo transition. Moreover, TOX has been shown to be involved in the development of NK and LTi cells. While its regulation remains unclear, TOX expression has been shown to be induced by calcineurin-mediated TCR signaling in thymocytes during positive selection.
Applications Reported: This TXRX10 antibody has been reported for use in immunoprecipitation and immunoblotting (WB).
Applications Tested: This TXRX10 antibody has been tested by immunoblotting of reduced cell lysate prepared from mouse thymocytes at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TOX (thymocyte selection-associated high mobility group (HMG) box protein) is a member of the HMG box family of DNA-binding proteins and likely plays a role in the regulation of T-cell development. It is a 526 amino acid nuclear protein and the expression of TOX is upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes. TOX-mediated positive selection is associated with up-regulation of RUNX3 and is calcineurin dependent. TOX-dependent transition to the CD4+CD8 stage is required for development of class II major histocompatibility complex-specific T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Thymocyte selection-associated high mobility group box protein TOX; thymocyte selection-associated HMG box; Thymus high mobility group box protein TOX; unnamed protein product
Gene Aliases: 1700007F02Rik; KIAA0808; TOX; TOX1
UniProt ID: (Human) O94900, (Mouse) Q66JW3
Entrez Gene ID: (Human) 9760, (Mouse) 252838
natural killer cell differentiation
CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment
NK T cell lineage commitment
leukocyte differentiation
chromatin organization
regulation of transcription from RNA polymerase II promoter
nervous system development
positive regulation of neuron projection development
cerebral cortex neuron differentiation
lymphocyte differentiation
positive regulation of natural killer cell differentiation
CD4-positive, alpha-beta T cell lineage commitment
CD8-positive, alpha-beta T cell lineage commitment
positive regulation of transcription from RNA polymerase II promoter
lymph node development
Peyer's patch development
regulation of positive thymic T cell selection
positive regulation of neural precursor cell proliferation
chromatin remodeling
regulation of transcription, DNA-templated
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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