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Phospho-RNA pol II CTD (Ser2) Monoclonal Antibody (3E10), eBioscience™
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FIGURE: 1 / 5
Phospho-RNA pol II CTD (Ser2) Antibody (14-9802-82) in ICC/IF
Immunofluorescence analysis of Phospho-POLR2A (Ser2) Monoclonal Antibody (Product # 14-9802-82) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 30 minutes at 37°C, and blocked with 10% Normal Goat Serum for 1 hour at room temperature. The cells were labeled with 1.25 µg/mL of Ph... View More
Product Details
14-9802-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rat
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
3E10
Immunogen
Ovalbumin-conjugated peptide corresponding to human RNA polymerase subunit B1 CTD phosphorylated at Ser2 (YS(p)PTSPSYSPTSPSC)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 3E10 monoclonal antibody recognizes a specific phosphorylated form of RNA polymerase II subunit B1 (RPB1). It detects phosphorylated serine at amino acid position 2 (Ser2) within the C-terminal heptapeptide (YSPTSPS) repeats of RPB1. Modifications within the C-terminal domain (CTD) of RPB1 recruit RNA processing factors that govern the transcription cycle; phosphorylation at Ser2 is associated with transcriptional elongation. The 3E10 immunogen was an ovalbumin-conjugated peptide corresponding to human RPB1 CTD phosphorylated at Ser2 (YS(p)PTSPSYSPTSPSC).
Applications Reported: This 3E10 antibody has been reported for use in western blot and immunocytochemistry.
Applications Tested: This 3E10 antibody has been tested by western blot of human, mouse, and rat cell lysates and immunocytochemistry of human cell lines. This may be used at less than or equal to 2.0 µg/mL for western blot and less than or equal to 1.25 µg/mL for immunocytochemistry.
Target Information
RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 3'-5' exoribonuclease; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.6; MGC75453; Pol II S2p; Polymerase (RNA II (DNA directed), large polypeptide; polymerase (RNA) II (DNA directed) polypeptide A; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; RNA; RNA POL2; RNA polymerase II; RNA polymerase II 1; RNA polymerase II C-terminal domain; RNA polymerase II carboxy-terminal domain; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; RPB220; RPO21; SUA8; unnamed protein product
Gene Aliases: 220kDa; hRPB220; hsRPB1; NEDHIB; POLR2; POLR2A; POLRA; RPB1; RPBh1; Rpii215; RpIILS; RPO2; Rpo2-1; RPOL2
UniProt ID: (Human) P24928, (Mouse) P08775
Entrez Gene ID: (Human) 5430, (Mouse) 20020, (Rat) 363633
magnesium ion binding
nucleic acid binding
DNA binding
RNA binding
DNA-directed RNA polymerase activity
RNA-directed RNA polymerase activity
protein binding
zinc ion binding
transferase activity
nucleotidyltransferase activity
hydrolase activity
kinase binding
ubiquitin protein ligase binding
RNA polymerase activity
metal ion binding
microfibril binding
promoter-specific chromatin binding
core promoter binding
RNA polymerase II activity
poly(A) RNA binding
molecular_function
DNA-directed RNA polymerase
transcription, RNA-templated
transcription, DNA-templated
DNA-templated transcription, termination
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
positive regulation of gene expression
positive regulation of RNA splicing
mRNA transcription from RNA polymerase II promoter
biological_process
response to organic cyclic compound
cellular response to oxygen levels
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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