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Invitrogen
CD94 Monoclonal Antibody (HP-3D9), APC, eBioscience™
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FIGURE: 1 / 1
CD94 Antibody (17-5094-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) FITC (Product # 11-0569) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Canine/Human CD94 APC (right). Cells in the lymphocyte gate were used for analysis.
Product Details
17-5094-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HP-3D9
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The HP-3D9 monoclonal antibody reacts with human CD94, a 70-kDa type II transmembrane glycoprotein. CD94 belongs to the C-type lectin superfamily and is expressed by NK cells, NKT cells, as well as subsets of CD8+ T cells and gamma delta T cells. This molecule is present as a heterodimer with members of the NKG2 family of receptors on the cell surface. CD94 dimerization with NKG2A results in an inhibitory receptor that suppresses NK cell function. In contrast, CD94 association with NKG2C and NKG2E leads to formation of an activating receptor complex that binds DAP12, an adapter protein that contains an ITAM motif. The CD94/NKG2 receptor complex binds the non-classical MHC Class Ib molecule HLA-E and plays important roles in adhesion and NK cell activation.
Crossblocking studies indicate that HP-3D9 and DX22 recognize the same epitope.
Applications Reported: This HP-3D9 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HP-3D9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Natural killer (NK) cells, like CD94, are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Three transcript variants encoding two different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CD94; CD94 antigen; Killer cell lectin-like receptor subfamily D member 1; killer cell lectin-like receptor subfamily D, member 1; KLRD1; KP43; natural killer cell receptor; Natural killer cells antigen CD94; NK cell receptor; unnamed protein product
Gene Aliases: CD94; KLRD1
UniProt ID: (Dog) Q38HS3, (Human) Q13241
Entrez Gene ID: (Dog) 611360, (Human) 3824
natural killer cell mediated immunity
negative regulation of T cell mediated cytotoxicity
stimulatory C-type lectin receptor signaling pathway
adaptive immune response
immune system process
cell surface receptor signaling pathway
regulation of natural killer cell activation
innate immune response
negative regulation of natural killer cell mediated cytotoxicity
positive regulation of natural killer cell mediated cytotoxicity
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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