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Human Hematopoietic Lineage Antibody Cocktail, eFluor™ 450, eBioscience™
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FIGURE: 1 / 1
Human Hematopoietic Lineage Antibody (22-7775-72) in Flow
Normal human peripheral blood cells were stained with Anti-Human CD34 FITC (Product # 11-0349-42), Anti-Human CD38 PE (Product # 12-0388-42), and the Human Hematopoietic Lineage eFluor® 450 Cocktail. Total viable cells were used for analysis. Cells in the right dot plot are gated on lineage negative events, depicted in the left histogram.
Product Details
22-7775-72
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
Class
Cocktail
Type
Antibody
Clone
RPA-2.10, OKT3, 61D3, CB16, HIB19, TULY56, HIR2
Conjugate
Excitation/Emission Max
405/445 nm
View spectra
Form
Liquid
Concentration
20 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The Human Hematopoietic Lineage eFluor™ 450 Cocktail contains eFluor™ 450-conjugated antibodies that can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their expression of cell surface antigens.
This cocktail contains the following antibodies:
Anti-Human CD2 (RPA-2.10) eFluor™ 450
Anti-Human CD3 (OKT3) eFluor™ 450
Anti-Human CD14 (61D3) eFluor™ 450
Anti-Human CD16 (CB16) eFluor™ 450
Anti-Human CD19 (HIB19) eFluor™ 450
Anti-Human CD56 (TULY56) eFluor™ 450
Anti-Human CD235a (HIR2) eFluor™ 450
Applications Reported: Human Hematopoietic Lineage eFluor™ 450 Cocktail has been reported for use in flow cytometric analysis.
Applications Tested: Human Hematopoietic Lineage eFluor™ 450 Cocktail has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Target Information
Hematopoietic Lineage antibodies can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their expression of cell surface antigens.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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