Western blot analysis of Apolipoprotein A-1 was performed by loading the indicated amounts of a recombinant human Apo A-1-hIgG1Fc fusion protein (Product # 10686-H02H-50) or a recombinant mouse Apo A-1-hIgG1Fc fusion protein (Product # 50918-M02H-50), and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a Novex® 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BOX ). Protei... View More

Mouse IgG Fc Cross-Adsorbed Secondary Antibody in Immunoprecipitation (IP)

Mouse IgG Fc Cross-Adsorbed Secondary Antibody in ELISA (ELISA)

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Product Details

31439

Applications

Tested Dilution

Western Blot (WB)

1:10,000-1:200,000

Immunohistochemistry (IHC)

1:500-1:5,000

Immunocytochemistry (ICC/IF)

1:500-1:5,000

ELISA (ELISA)

1:5,000-1:100,000

Immunoprecipitation (IP)

1:10,000-1:20,000

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

HRP HRP HRP

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 15mg/mL BSA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_228292

Target

IgG Fc

Cross Adsorption

Against human, bovine and horse serum proteins

Antibody Form

Whole Antibody

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

Product # 31439 has been successfully used in Western blot, and ICC applications.

Antibody Specificity: This antibody reacts with heavy chains on mouse IgG but not with the light chains on most mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins or mouse IgM. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins. However, this antibody may cross-react with immunoglobulins from other species.

Restoration and Storage: Store product at 4°C until opened. Restore with 1.5 mL distilled water (0.8 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.

To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.

To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.

Country of Origin: USA

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Goat anti-Mouse IgG Fc Cross-Adsorbed Secondary Antibody, HRP

Mouse IgG Fc Cross-Adsorbed Secondary Antibody (31439) in WB