Search
Invitrogen
Prolactin Receptor Monoclonal Antibody (1A2B1)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 37
Prolactin Receptor Antibody (35-9200) in ICC/IF
Immunofluorescence analysis of Prolactin Receptor was performed using 70% confluent log phase SW40 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Prolactin Receptor (ECD) (1A2B1) Monoclonal Antibody (Product # 35-9200) at 2µg/mL in 0.1% BSA and incub... View More
Product Details
35-9200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Hamster,
Human,
Mouse
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
1A2B1
Immunogen
Recombinant human prolactin receptor protein containing the extracellular domain (ECD).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with mouse and rat and reacts with the long form of the human prolactin receptor (hPRLr) protein at ~85-90 kDa, and may also identify the human prolactin receptor intermediate and delta S1 isoforms by western blotting. On immunoprecipitation, this antibody strongly recognizes only the long form of hPRLr. During development reactivity was confirmed with human T-47D breast carcinoma cells.
Target Information
Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor (PRLR), a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRLR consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cytokine receptor; cytokine receptor; PRLRSFA; cytokine receptor; PRLRSFB; delta-S1 PRLr; hPRL receptor; Prl R; PRL-R; Prolactin receptor; secreted prolactin binding protein; unnamed protein product
Gene Aliases: HPRL; hPRLrI; MFAB; PRLR; RI-PRLR
UniProt ID: (Human) P16471
Entrez Gene ID: (Human) 5618
steroid biosynthetic process
activation of transmembrane receptor protein tyrosine kinase activity
JAK-STAT cascade
embryo implantation
lactation
positive regulation of cell proliferation
response to bacterium
cytokine-mediated signaling pathway
regulation of cell adhesion
regulation of epithelial cell differentiation
positive regulation of B cell proliferation
prolactin signaling pathway
activation of Janus kinase activity
negative regulation of apoptotic process
mammary gland epithelial cell differentiation
prostate gland growth
mammary gland alveolus development
mammary gland epithelium development
cellular response to granulocyte macrophage colony-stimulating factor stimulus
positive regulation of cold-induced thermogenesis
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support