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Invitrogen
NR5A1 Monoclonal Antibody (N1665)
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FIGURE: 1 / 10
NR5A1 Antibody (434200) in WB
Western blot was performed using NR5A1 Monoclonal Antibody (Product # 434200) and a 50 kDa band corresponding to NR5A1 was observed in the cell line and tissues tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of Hep G2 (Lane 1), Hek-293 (Lane 2), tissue extracts of Mouse Ovary (Lane 3), Mouse Heart (Lane 4) and Mouse Prostate (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blo... View More
Product Details
434200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N1665
Immunogen
BALB/c mouse immunized with E.coli-expressed recombinant human SF-1 (218-461aa).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
physiological saline
Contains
0.1% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody specifically recognizes human SF-1 and cross reacts with mouse and rat SF-1.
Target Information
Steroidogenic factor-1 (SF-1) is an orphan nuclear receptor involved in organ development and differentiation of the endocrine system. SF-1 has been shown to be essential for fetal development of the hypothalamus, pituitary gland, gonads, and the adrenal gland. SF-1 has been shown to promote transcriptional activity of many steroidogenic enzymes from the cytochrome P450 family. SF-1 contains two activation function domains, designated AF1 and AF2, common to many nuclear steroid receptors which have been shown to be necessary for interaction with other nuclear receptors as well as coactivators and corepressors including steroid receptor coactivator-1 (SRC-1), glucocorticoid receptor interacting protein 1 (GRIP1), cAMP response element binding protein (CREB) binding protein (CBP) and silencing mediator of retinoid acid and thyroid hormone receptor (SMRT).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: adrenal 4 binding protein; Adrenal 4 binding protein (AD4BP); Adrenal 4-binding protein; ELP; Embryonal long terminal repeat-binding protein; Embryonal LTR-binding protein; fushi tarazu 1 factor homolog; Fushi tarazu factor homolog 1; Fushi tarazu factor homolog 1 (FTZ1); nuclear receptor AdBP4; Nuclear receptor subfamily 5 group A member 1; Nuclear receptor subfamily 5 group A member 1 (NR5A1); putative new variant; SF-1; Steroid hormone receptor Ad4BP; Steroid hydroxylase positive regulator; Steroidogenic factor 1; Steroidogenic factor 1 (SF1); steroidogenic factor 1 nuclear receptor; steroidogenic factor-1; STF-1; STF1
Gene Aliases: AD4BP; Ad4BP/SF-1; ELP; ELP-3; Ftz-F1; FTZ1; FTZF1; hSF-1; NR5A1; POF7; SF-1; SF1; SPGF8; SRXX4; SRXY3
UniProt ID: (Human) Q13285, (Mouse) P33242, (Rat) P50569
Entrez Gene ID: (Human) 2516, (Mouse) 26423, (Rat) 83826
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription cofactor binding
DNA binding
chromatin binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
phospholipid binding
zinc ion binding
lipid binding
enzyme binding
sequence-specific DNA binding
metal ion binding
sequence-specific double-stranded DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
steroid hormone receptor activity
C4 zinc finger nuclear receptor
luteinization
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
sex determination
primary sex determination
male gonad development
female gonad development
hormone-mediated signaling pathway
tissue development
positive regulation of gene expression
male sex determination
adrenal gland development
intracellular receptor signaling pathway
Leydig cell differentiation
hormone metabolic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of steroid biosynthetic process
maintenance of protein location in nucleus
Sertoli cell differentiation
response to gonadotropin-releasing hormone
calcineurin-mediated signaling
positive regulation of male gonad development
negative regulation of female gonad development
transcription, DNA-templated
multicellular organism aging
reproductive process
cell differentiation
steroid hormone mediated signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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