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Invitrogen
STAT3 Polyclonal Antibody
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FIGURE: 1 / 1
STAT3 Antibody (44-364G) in WB
Western blot analysis was performed on whole cell extracts (20 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), A431 (Lane 3), A549 (Lane 4), and SKBR3 (Lane 5). The blots were probed with Anti- STAT3 Rabbit polyclonal antibody (Product # 44-364G, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG HRP conjugate (Product # G-21234, 1:10,000 dilution). A ~ 90 kDa band corresponding to STAT3 was observed across cell lines tested.
Product Details
44-364G
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a synthetic peptide corresponding to residues near the carboxyl-terminal sequence of human STAT3
Conjugate
Form
Liquid
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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