Search

  • Order Status
  • Quick Order

Invitrogen

Phospho-FAK (Tyr397) Monoclonal Antibody (141-9)

44 Published Figures
62 References
View all (108) FAK antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
Phospho-FAK (Tyr397) Monoclonal Antibody (141-9)

Product Details

44-625G

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
View 39 publications 39 publications

Immunohistochemistry (IHC)

-
View 10 publications 10 publications

Immunohistochemistry (Frozen) (IHC (F))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent
View 10 publications 10 publications

Immunoprecipitation (IP)

-
View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Chicken, Fruit fly, Human, Mouse, Rat, Xenopus

Published species

Fish, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

141-9

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 397. The sequence is conserved in mouse, rat, chicken and frog.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533702

Product Specific Information

This antibody will cross-react with the corresponding autophosphorylation site on Proline-rich/Ca2+- activated tyrosine kinase (Pyk2), [pY402]. FAK [pY397] polyclonal antibody (Cat. No. 44-624G) does not cross-react with Pyk2.

The antibody has been used in western blotting. Previous lots of this antibody have been used in immunocytochemistry. For western blotting applications, we recommend using the antibody at a 1:1,000 starting dilution. For immunofluorecence staining applications, we recommend a 1:50 starting dilution. Positive control used: Primary chicken embryo fibroblasts expressing human FAK and plated on fibronectin.

We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20° C. For shipment or short-term storage (up to one week), 2-8° C is sufficient. Expires one year from date of receipt when stored as instructed.

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CG10023-PA; CG10023-PB; CG10023-PC; CG10023-PD; CG10023-PF; CG10023-PG; EC 2.7.10.2; FADK 1; FAK-Del33; exon 33 deletion splicing mutant; Fak-PA; Fak-PB; Fak-PC; Fak-PD; Fak-PF; Fak-PG; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase homolog; focal adhesion kinase pp125FAK; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; p41/p43FRNK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein tyrosine kinase 2; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; tyrosine kinase; unnamed protein product

View more View less

Gene Aliases: CG10023; CT28129; DFAK; DFak56; Dmel\CG10023; Dmel_CG10023; DmFAK; FADK; FADK 1; FAK; FAK1; Fak56; Fak56D; FAK65D; FRNK; Kiaa4203; mKIAA4203; p125FAK; pFAK; pp125FAK; PPP1R71; PTK2; XELAEV_18032408mg; XPFAK

View more View less

UniProt ID: (Human) Q05397, (Mouse) P34152, (Rat) O35346, (Xenopus) Q91738

View more View less

Entrez Gene ID: (Fruit fly) 37233, (Human) 5747, (Chicken) 396416, (Mouse) 14083, (Rat) 25614, (Xenopus) 399286

View more View less

Function(s)
non-membrane spanning protein tyrosine kinase activity ATP binding nucleotide binding actin binding protein kinase activity protein tyrosine kinase activity protein tyrosine phosphatase activity integrin binding protein binding JUN kinase binding kinase activity transferase activity protein kinase binding protein phosphatase binding SH2 domain binding molecular function activator activity protease binding calcium-dependent cysteine-type endopeptidase activity receptor binding identical protein binding signal transducer activity transferase activity, transferring phosphorus-containing groups protein complex binding phosphatidylinositol 3-kinase binding
Process(es)
MAPK cascade microtubule cytoskeleton organization angiogenesis blood vessel development vasculogenesis neuron migration regulation of protein phosphorylation positive regulation of protein phosphorylation protein phosphorylation movement of cell or subcellular component cell adhesion signal complex assembly epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway JNK cascade multicellular organism development positive regulation of cell proliferation regulation of cell shape regulation of epithelial cell migration positive regulation of phosphatidylinositol 3-kinase signaling positive regulation of smooth muscle cell migration phosphorylation peptidyl-tyrosine phosphorylation central nervous system neuron axonogenesis negative regulation of cell-cell adhesion regulation of cell adhesion extracellular matrix organization positive regulation of cell growth positive regulation of cell migration negative regulation of cell migration cellular chloride ion homeostasis regulation of cell adhesion mediated by integrin peptidyl-tyrosine autophosphorylation establishment of nucleus localization regulation of cell proliferation negative regulation of apoptotic process endothelial cell migration innate immune response regulation of osteoblast differentiation positive regulation of cell adhesion positive regulation of protein kinase activity positive regulation of vasodilation negative regulation of organ growth protein autophosphorylation ephrin receptor signaling pathway positive regulation of smooth muscle cell proliferation positive regulation of phagocytosis negative regulation of axonogenesis positive regulation of synaptic transmission regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of synapse assembly positive regulation of glial cell proliferation growth hormone receptor signaling pathway cellular response to transforming growth factor beta stimulus regulation of substrate adhesion-dependent cell spreading positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of anoikis placenta development regulation of cell-matrix adhesion heart morphogenesis axon guidance regulation of endothelial cell migration positive regulation of epithelial cell migration positive regulation of epithelial to mesenchymal transition positive regulation of macrophage chemotaxis positive regulation of fibroblast migration cell migration establishment of cell polarity cell differentiation detection of muscle stretch netrin-activated signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis regulation of GTPase activity regulation of cell differentiation vascular endothelial growth factor receptor signaling pathway cell motility regulation of multicellular organismal process regulation of cytoskeleton organization positive regulation of wound healing vascular endothelial cell response to oscillatory fluid shear stress positive regulation of macrophage proliferation cytoskeleton organization response to mechanical stimulus response to glucose response to organic substance response to organonitrogen compound response to organic cyclic compound response to estradiol response to drug fat cell differentiation response to arsenic-containing substance proteolysis actin filament organization transmembrane receptor protein tyrosine kinase signaling pathway response to pH negative regulation of cell-substrate adhesion pyramidal neuron migration negative regulation of protein autophosphorylation positive regulation of protein binding response to muscle stretch wound healing, spreading of cells positive regulation of focal adhesion assembly angiogenesis involved in wound healing positive regulation of protein tyrosine kinase activity positive regulation of substrate-dependent cell migration, cell attachment to substrate negative regulation of receptor recycling negative regulation of synaptic growth at neuromuscular junction
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support