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Invitrogen
Phospho-Paxillin (Tyr31) Polyclonal Antibody
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FIGURE: 1 / 13
Phospho-Paxillin (Tyr31) Antibody (44-720G) in ICC/IF
Immunofluorescence analysis of Phospho-Paxillin pTyr31 was done on 70% confluent log phase NIH/3T3 cells were treated with 50 ng of PDGF for 10 minutes and fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Paxillin (Tyr31) Rabbit Polyclonal Antibody (Product # 44-720... View More
Product Details
44-720G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Pig,
Rat,
Tag
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human paxillin that contains tyrosine 31.
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ16691; hCG_1778014; PAX-1; PAXI; Paxillin; paxillin alpha; testicular tissue protein Li 134; unnamed protein product
Gene Aliases: AW108311; AW123232; Pax; PXN
UniProt ID: (Human) P49023, (Mouse) Q8VI36
Entrez Gene ID: (Human) 5829, (Mouse) 19303
protein binding
beta-catenin binding
vinculin binding
protein phosphatase binding
neuropilin binding
metal ion binding
integrin binding
zinc ion binding
protein kinase binding
receptor signaling complex scaffold activity
protein complex binding
BH4 domain binding
actin or actin-binding cytoskeletal protein
cell adhesion
signal transduction
signal complex assembly
transforming growth factor beta receptor signaling pathway
cell migration
substrate adhesion-dependent cell spreading
cellular response to reactive oxygen species
endothelial cell migration
positive regulation of stress fiber assembly
growth hormone receptor signaling pathway
activation of MAPK activity
movement of cell or subcellular component
cytoskeleton organization
cell-matrix adhesion
integrin-mediated signaling pathway
regulation of cell shape
peptidyl-tyrosine phosphorylation
lamellipodium assembly
positive regulation of protein kinase activity
focal adhesion assembly
branching morphogenesis of an epithelial tube
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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