Search
Invitrogen
SDHA Monoclonal Antibody (2E3GC12FB2AE2)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 70
SDHA Antibody (459200) in ICC/IF
Immunofluorescent analysis of SDHA in human embryonic lung-derived fibroblasts using an SDHA Monoclonal antibody (Product # 459200) at a concentration of 0.2 µg/mL. Cultured human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with an SDHA Monoclonal antibody (Product # 459200) at a concentration of 0.2 µg/mL, followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody at a concentration of 2 µg/mL.
.jpg?time=20240507)
.jpg?time=20240507?time=20240507 "SDHA Antibody (459200) in WB")
Product Details
459200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Published species
Dog,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
2E3GC12FB2AE2
Immunogen
Full length native protein (purified) corresponding to Cow SDHA. Purified mitochondrial complex II (Cow).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
IgG fraction
Storage buffer
HEPES buffered saline, pH 7.5
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
ICC application requires heat-induced antigen retrieval where aldehydes are used as fixatives. Use 20min incubation at 90-100°C in 0.1 M Tris/HCl pH 9.5 with 5% urea (wt/vol).
Positive control: Human heart mitochondria.
Target Information
This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: flavoprotein; Flavoprotein subunit of complex II; Fp; Malate dehydrogenase [quinone] flavoprotein subunit; mitochondrial succinate dehydrogenase complex subunit A; SDHA; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; succinate dehydrogenase complex subunit A; succinate dehydrogenase complex, subunit A, flavoprotein (Fp); succinate dehydrogenase flavoprotein subunit A; unnamed protein product
Gene Aliases: 1500032O14Rik; 2310034D06Rik; 4921513A11; C81073; CMD1GG; FP; MC2DN1; NDAXOA; PGL5; PPGL5; SDH1; SDH2; SDHA; SDHF; SDHFP1
UniProt ID: (Bovine) P31039, (Human) P31040, (Rat) Q920L2, (Mouse) Q8K2B3
Entrez Gene ID: (Bovine) 281480, (Human) 6389, (Rat) 157074, (Mouse) 66945
succinate dehydrogenase activity
succinate dehydrogenase (ubiquinone) activity
protein binding
electron carrier activity
oxidoreductase activity
oxidoreductase activity, acting on the CH-CH group of donors
flavin adenine dinucleotide binding
dehydrogenase
oxidoreductase
metabolite interconversion enzyme
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support