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NeoBiotechnologies

Nucleophosmin Monoclonal Antibody (NPM1, 3286)

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Nucleophosmin Monoclonal Antibody (NPM1, 3286)

Product Details

4869-MSM6-P1ABX

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL
-

Immunocytochemistry (ICC/IF)

1-2 µg/mL
-

Flow Cytometry (Flow)

1-2 µg/10^6 cells
-

Peptide Array (Array)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

NPM1, 3286

Immunogen

Recombinant human NPM1 protein fragment (aa185-287)
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Antibody is tested in direct ELISA (Use Ab at 2-4 µg/mL for coating.

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm. NPM1 has been shown to bind nucleic acid, prevent protein aggregation via its chaperon activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM1 is associated with the centrosome, and is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Due to the NPM1 gene interaction with several tumor-associated chromosome translocations, NPM1 is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, NPM1 is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins. Further, NPM1 is also known to sequester the tumor suppressor RF in the nucleolus, protecting it from degradation until it is necessary. Dysfunction of the NPM1 protein is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B23 antibody; deletion in NPMc- AML; MGC104254; mNPM antibody; mNPM1 antibody; mutation A in NPMc+ AML; mutation B in NPMc+ AML; mutation C in NPMc+ AML; mutation D in NPMc+ AML; mutation E in NPMc+ AML; mutation F in NPMc+ AML; NO38 antibody; NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23 numatrin) isoform NPM1_1; nucleophosmin (nucleolar phosphoprotein B23 numatrin) isoform NPM1_2; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin/nucleoplasmin family, member 1; Numatrin; numatrin antibody; testicular tissue protein Li 128; unnamed protein product

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Gene Aliases: B23; NPM; NPM1

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UniProt ID: (Human) P06748

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Entrez Gene ID: (Human) 4869

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Function(s)
core promoter sequence-specific DNA binding nucleic acid binding chromatin binding transcription coactivator activity RNA binding protein kinase inhibitor activity protein binding rRNA binding protein kinase binding Tat protein binding histone binding identical protein binding protein homodimerization activity ribosomal large subunit binding ribosomal small subunit binding NF-kappaB binding unfolded protein binding binding, bridging DNA-binding transcription factor binding
Process(es)
ribosomal large subunit export from nucleus ribosomal small subunit export from nucleus regulation of cell growth DNA repair nucleosome assembly chromatin remodeling protein import into nucleus cell volume homeostasis intracellular protein transport nucleocytoplasmic transport centrosome cycle signal transduction protein localization positive regulation of cell proliferation negative regulation of cell proliferation positive regulation of biosynthetic process positive regulation of macromolecule metabolic process posttranscriptional regulation of gene expression negative regulation of gene expression regulation of centrosome duplication positive regulation of centrosome duplication negative regulation of centrosome duplication macrophage differentiation positive regulation of protein ubiquitination regulation of protein stability regulation of endodeoxyribonuclease activity cellular response to UV regulation of cell proliferation ribosome biogenesis ribosome assembly ribosomal large subunit biogenesis ribosomal small subunit biogenesis negative regulation of apoptotic process regulation of DNA damage response, signal transduction by p53 class mediator negative regulation of protein kinase activity by regulation of protein phosphorylation positive regulation of translation positive regulation of transcription, DNA-templated positive regulation of nucleobase-containing compound metabolic process positive regulation of transcription from RNA polymerase II promoter regulation of centriole replication negative regulation of mRNA splicing, via spliceosome protein stabilization positive regulation of NF-kappaB transcription factor activity regulation of cell cycle regulation of endoribonuclease activity regulation of eIF2 alpha phosphorylation by dsRNA cellular senescence regulation of mRNA stability involved in cellular response to UV positive regulation of cell cycle G2/M phase transition positive regulation of protein localization to nucleolus
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