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Invitrogen
CXCL10 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
CXCL10 Antibody (500-P129BT-1MG) in WB
Western Blot: To detect mIP-10 by Western Blot analysis CXCL10 Polyclonal Antibody, Biotin (Product # 500-P129BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mIP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P129BT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine IP-10 (CXCL10)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: IPLARTVRCN CIHIDDGPVR MRAIGKLEII PASLSCPRVE IIATMKKNDE QRCLNPESKT IKNLMKAFSQ KRSKRAP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IP-10 (CXCL10). Anti-Murine IP-10 (CXCL10)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine IP-10 (CXCL10) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IP-10 (CXCL10) (500-P129) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IP-10 (CXCL10).
Western Blot: To detect mIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mIP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P129BT-1MG will be provided as 2 x 500 µg
Target Information
IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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