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Invitrogen
IGF1 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
IGF1 Antibody (500-P157GBT-1MG) in WB
Western Blot: To detect mIGF-I by Western Blot analysis IGF1 Polyclonal Antibody, Biotin (Product # 500-P157GBT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mIGF-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P157GBT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine IGF-I
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine IGF-I. Anti-Murine IGF-I-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mIGF-I by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IGF-I (500-P157G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mIGF-I.
Western Blot: To detect mIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mIGF-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P157GBT-1MG will be provided as 2 x 500 µg
Target Information
IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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