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Invitrogen

14-3-3 Pan Polyclonal Antibody

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12 Published Figures
14 References
View all (7) 14-3-3 Pan antibodies

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14-3-3 Pan Polyclonal Antibody

Product Details

51-0700

Applications
Tested Dilution
Publications

Western Blot (WB)

2 µg/mL
View 11 publications 11 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL
View 2 publications 2 publications

ELISA (ELISA)

0.1-1.0 µg/mL
-
Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat, Xenopus

Published species

Bovine, Fruit fly, Human, Mouse, Plant

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 20 amino acid synthetic peptide derived from the N-terminus of the human 14-3-3 beta/alpha protein.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Amount

200 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533894

Product Specific Information

This antibody is broadly reactive with members of the 14-3-3 family of proteins (~29-32 kDa). With the exception of a single conserved amino acid change, this sequence is identical in the delta and zeta isoforms. Three and four conserved amino acid differences are found in the tau and gamma isoforms, respectively.

Based on the sequence homology to the immunizing peptide, this the antibody could recognize the protein from C. elegans.

Target Information

At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and, through interaction with Bad, prevents apoptosis. Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf and the IGF-1 receptor. Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be quite useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 14-3-3 alpha; 14-3-3 beta; 14-3-3 delta; 14-3-3 epsilon; 14-3-3 gamma; 14-3-3 protein beta; 14-3-3 protein beta-subtype; 14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha-A; 14-3-3 protein beta/alpha-B; 14-3-3 protein epsilon; 14-3-3 protein gamma; 14-3-3 protein gamma subtype; 14-3-3 protein gamma-A; 14-3-3 protein gamma-B; 14-3-3 protein gamma-subtype; 14-3-3 protein sigma; 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; 14-3-3 protein zeta; 14-3-3 protein zeta/delta; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 sigma protein; 14-3-3 tau; 14-3-3 theta; 14-3-3 zeta; 14-3-3d; 14-3-3E; 3-monooxgenase/tryptophan 5-monooxgenase activation protein, gamma polypeptide; 3-monooxgenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide; 3-monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide; 3-monooxygenase/tryptophan 5-monooxgenase activation protein gamma polypeptide; 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide; epididymis luminal protein 2; epididymis luminal protein 4; epididymis secretory protein Li 1; epididymis secretory protein Li 3; epididymis secretory protein Li 93; Epithelial cell marker protein 1; Factor activating exoenzyme S; FAS; HS1; KCIP-1; mitochondrial import stimulation factor (MSF) L subunit; Mitochondrial import stimulation factor L subunit; Mitochondrial import stimulation factor S1 subunit; MSF L; phospholipase A2; Prepronerve growth factor RNH-1; Protein 1054; Protein HS1; Protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; protein phosphatase 1, regulatory subunit 170; protein tau; protein, theta; SEZ-2; SFN; signal transduction phosphoprotein; Stratifin; tryosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein gamma; tryosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein gamma polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein, gamma polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, sigma polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein, epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein gamma polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, gamma polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, theta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; unnamed protein product; YWHAG

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Gene Aliases: 1110013I11Rik; 1300003C17Rik; 14-3-3; 14-3-3-zeta; 14-3-3beta; 14-3-3E; 14-3-3G; 14-3-3GAMMA; 14-3-3t; 14-3-3z; 14-3-3zeta; 1C5; 2700028P07Rik; AA409740; AI596267; AL022924; AU019196; AU020854; AU021156; D7Bwg1348e; DEE56; EIEE56; Er; GW128; HEL-S-1; HEL-S-3; HEL-S-93; HEL2; HEL4; HME1; HS1; KCIP-1; MDCR; MDS; Mkrn3; Mme1; Msfs1; POPCHAS; PPP1R170; R74690; SFN; XELAEV_18012695mg; XELAEV_18015318mg; XELAEV_18043238mg; XELAEV_18045999mg; YWHAA; YWHAB; ywhab-a; ywhab-b; YWHAD; YWHAE; YWHAG; ywhag-a; ywhag-b; YWHAQ; YWHAS; YWHAZ

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UniProt ID: (Bovine) Q0VC36, (Human) P31947, (Mouse) O70456, (Bovine) P68250, (Human) P31946, (Bovine) P62261, (Human) P62258, (Human) P61981, (Bovine) Q3SZI4, (Human) P27348, (Human) P63104, (Bovine) P63103, (Mouse) Q9CQV8, (Rat) P35213, (Mouse) P62259, (Rat) P62260, (Mouse) P61982, (Rat) P61983, (Rat) P68255, (Mouse) P68254, (Mouse) P63101, (Rat) P63102, (Xenopus) Q5XHK2, (Xenopus) Q8AVQ3, (Xenopus) Q6PCG0, (Xenopus) Q6NRY9

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Entrez Gene ID: (Bovine) 528453, (Human) 2810, (Mouse) 55948, (Rat) 313017, (Bovine) 286863, (Human) 7529, (Bovine) 282125, (Human) 7531, (Human) 7532, (Bovine) 286862, (Bovine) 768311, (Human) 10971, (Human) 7534, (Bovine) 287022, (Mouse) 54401, (Rat) 56011, (Mouse) 22627, (Rat) 29753, (Mouse) 22628, (Rat) 56010, (Rat) 25577, (Mouse) 22630, (Mouse) 22631, (Rat) 25578, (Xenopus) 399276, (Xenopus) 380457, (Xenopus) 379724, (Xenopus) 432005, (Xenopus) 100505434

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Function(s)
protein kinase binding identical protein binding phosphoprotein binding protein binding protein kinase C inhibitor activity cadherin binding phosphoserine binding protein sequestering activity protein domain specific binding molecular_function protein kinase inhibitor activity protein phosphatase inhibitor activity enzyme binding histone deacetylase binding macromolecular complex binding RNA binding calcium channel regulator activity potassium channel regulator activity calcium channel inhibitor activity protein phosphatase binding MHC class II protein complex binding ubiquitin protein ligase binding signaling adaptor activity ion channel binding protein heterodimerization activity scaffold protein binding molecular function inhibitor activity protein kinase C binding insulin-like growth factor receptor binding receptor tyrosine kinase binding phosphorylation-dependent protein binding 14-3-3 protein binding DNA-binding transcription factor binding transcription corepressor activity protein C-terminus binding protein complex binding poly(A) RNA binding actin binding enzyme regulator activity protein N-terminus binding transcription factor binding scaffold/adaptor protein transporter
Process(es)
regulation of cyclin-dependent protein serine/threonine kinase activity release of cytochrome c from mitochondria keratinocyte development intrinsic apoptotic signaling pathway in response to DNA damage regulation of epidermal cell division negative regulation of keratinocyte proliferation positive regulation of cell growth keratinization negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of epidermal cell differentiation positive regulation of protein export from nucleus establishment of skin barrier negative regulation of transcription from RNA polymerase II promoter negative regulation of protein kinase activity protein export from nucleus signal transduction protein localization regulation of cell-cell adhesion keratinocyte differentiation regulation of protein localization defense response to bacterium skin development keratinocyte proliferation positive regulation of cell adhesion negative regulation of innate immune response cytolysis in other organism regulation of cell cycle stem cell proliferation cAMP/PKA signal transduction non-canonical inflammasome complex assembly negative regulation of protein localization to plasma membrane positive regulation of cellular protein localization negative regulation of stem cell proliferation biological_process protein targeting negative regulation of G-protein coupled receptor protein signaling pathway negative regulation of protein import into nucleus negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter MAPK cascade protein localization to nucleus cellular response to heat neuron migration cytoplasmic pattern recognition receptor signaling pathway regulation of heart rate by hormone ER to Golgi vesicle-mediated transport Golgi to plasma membrane transport regulation of mitotic cell cycle substantia nigra development hippocampus development cerebral cortex development cellular potassium ion homeostasis negative regulation of toll-like receptor signaling pathway positive regulation of toll-like receptor signaling pathway toll-like receptor 4 signaling pathway hippo signaling positive regulation of hippo signaling intracellular signal transduction proteasome-mediated ubiquitin-dependent protein catabolic process regulation of cytosolic calcium ion concentration regulation of membrane repolarization protein localization to endoplasmic reticulum protein K11-linked ubiquitination membrane repolarization during cardiac muscle cell action potential regulation of heart rate by cardiac conduction regulation of potassium ion transmembrane transport negative regulation of calcium ion export across plasma membrane cytoplasmic translation negative regulation of T cell mediated immune response to tumor cell positive regulation of T cell mediated immune response to tumor cell regulation of signal transduction positive regulation of cell-cell adhesion SCF-dependent proteasomal ubiquitin-dependent protein catabolic process cellular response to nutrient levels cellular response to insulin stimulus cellular response to amino acid starvation TORC1 signaling cellular response to glucose starvation regulation of neuron differentiation negative regulation of translational initiation positive regulation of translational initiation regulation of synaptic plasticity protein stabilization negative regulation of T cell activation positive regulation of T cell activation protein localization to lysosome protein K6-linked ubiquitination negative regulation of TORC1 signaling positive regulation of TORC1 signaling small GTPase mediated signal transduction negative regulation of ion transmembrane transport angiogenesis respiratory system process protein phosphorylation lysosome organization synaptic target recognition lung development regulation of protein stability tube formation negative regulation of apoptotic process regulation of programmed cell death establishment of Golgi localization ERK1 and ERK2 cascade regulation of ERK1 and ERK2 cascade regulation of synapse maturation Golgi reassembly antibacterial innate immune response negative regulation of protein localization to nucleus regulation of lysosome organization negative regulation of protein dephosphorylation positive regulation of catalytic activity cytoplasmic sequestering of protein protein heterooligomerization regulation of potassium ion transmembrane transporter activity negative regulation of peptidyl-serine dephosphorylation negative regulation of protein serine/threonine kinase activity regulation of catalytic activity histamine secretion by mast cell protein targeting to mitochondrion regulation of cell death response to drug
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