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Rockland

Phospho-BLM (Ser527) Polyclonal Antibody

View all (25) Blooms Syndrome antibodies

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Product Details

600-401-J58

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (IHC)

Assay-dependent
-

ELISA (ELISA)

1: 10,000
-

Immunoprecipitation (IP)

1 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-BLM pS527 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human Bloom syndrome protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 30% glycerol, 0.15M NaCl

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-BLM pS527 is directed against the phosphorylated form of human S527 residue. A BLAST analysis was used to suggest cross-reactivity with human based on 100% sequence homology. Cross-reactivity with BLM pS527 from other sources has not been determined.

Target Information

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bloom syndrome protein; Bloom syndrome RecQ like helicase; Bloom syndrome, RecQ helicase-like; DNA 3'-5' helicase BLM; DNA helicase, RecQ-like type 2; MGC126616; MGC131618; MGC131620; RecQ protein-like 3; RecQ-like DNA helicase BLM; RecQ2; unnamed protein product

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Gene Aliases: BLM; BS; MGRISCE1; RECQ2; RECQL2; RECQL3

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UniProt ID: (Human) P54132

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Entrez Gene ID: (Human) 641

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Function(s)
nucleotide binding four-way junction DNA binding Y-form DNA binding bubble DNA binding p53 binding nucleic acid binding DNA binding DNA helicase activity single-stranded DNA binding helicase activity protein binding ATP binding DNA-dependent ATPase activity zinc ion binding four-way junction helicase activity hydrolase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides isomerase activity ATPase activity identical protein binding protein homodimerization activity 3'-5' DNA helicase activity metal ion binding G-quadruplex DNA binding forked DNA-dependent helicase activity telomeric D-loop binding telomeric G-quadruplex DNA binding 8-hydroxy-2'-deoxyguanosine DNA binding DNA/DNA annealing activity
Process(es)
regulation of cyclin-dependent protein serine/threonine kinase activity telomere maintenance double-strand break repair via homologous recombination DNA double-strand break processing DNA replication DNA repair DNA recombination cellular response to DNA damage stimulus mitotic G2 DNA damage checkpoint response to X-ray replication fork processing telomere maintenance via semi-conservative replication DNA geometric change positive regulation of transcription, DNA-templated negative regulation of DNA recombination protein oligomerization protein homooligomerization negative regulation of cell division telomeric D-loop disassembly resolution of recombination intermediates cellular response to ionizing radiation cellular response to hydroxyurea cellular response to camptothecin t-circle formation
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