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Invitrogen
Vinculin Recombinant Rabbit Monoclonal Antibody (42H89L44)
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FIGURE: 1 / 40
Vinculin Antibody (700062) in WB
Knockout of Vinculin was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR948199_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Vinculin was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) andHeLa Vinculin KO (Lane 3) membrane enriched extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Produ... View More
Product Details
700062
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Deer,
Fruit fly,
Hamster,
Human,
Mouse,
Non-human primate,
Rabbit
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
42H89L44
Immunogen
A recombinant protein corresponding to amino acids 408-591 of P18206.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
This antibody reacts with Human Vinculin. Based on sequence similarity, reactivity to chimpanzee, Rhesus monkey, swine, equine, mouse, rat, bovine, and chicken is expected. Immunogen sequence IRGALAEARKIAELCDDPKERDDILRSLGEI SALTSKLADLRRQGKGDSPEARALAKQV ATALQNLQTKTNRAVANSRPAKAAVHLE GKIEQAQRWIDNPTVDDRGVGQAAIRGL VAEGHRLANVMMGPYRQDLLAKCDRVD QLTAQLADLAARGEGESPQARALASQLQ DSLKDLKARMQEAM. Test material for western blotting/immunofluorescence: HeLa cells.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis luminal protein 114; epididymis secretory sperm binding protein; meta-vinculin; Metavinculin; MV; RP11-178G16.3; unnamed protein product; vinc; Vinculin
Gene Aliases: 9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; HEL114; MV; MVCL; VCL; VINC
UniProt ID: (Human) P18206, (Mouse) Q64727
Entrez Gene ID: (Human) 7414, (Mouse) 22330
morphogenesis of an epithelium
cell adhesion
cell-matrix adhesion
lamellipodium assembly
regulation of cell migration
negative regulation of cell migration
adherens junction assembly
protein localization to cell surface
maintenance of blood-brain barrier
apical junction assembly
axon extension
regulation of focal adhesion assembly
platelet aggregation
epithelial cell-cell adhesion
regulation of establishment of endothelial barrier
regulation of protein localization to cell-cell adherens junction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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