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Invitrogen
Securin Recombinant Rabbit Monoclonal Antibody (19H16L48)
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FIGURE: 1 / 13
Securin Antibody (700791) in ICC/IF
Immunofluorescent analysis of Securin in HeLa cells using a Securin recombinant rabbit monoclonal antibody (Product # 700791) at a dilution of 2 µg/mL followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Cells were fixed using 4% paraformaldehyde. The cytoplasmic localization of Securin is shown in green, while nuclei were stained using SlowFade GOLD with DAPI (Product # S36938), shown in blue.
Product Details
700791
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
19H16L48
Immunogen
A recombinant protein corresponding to amino acids 1-202.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with bovine based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: endogenous cardiac regeneration-associated regulator; Esp1-associated protein; ESP1-associated protein 1; HTTG; oncoprotein; MGC126883; MGC138276; pituitary tumor-transforming 1; Pituitary tumor-transforming gene 1 protein; PTTG1; Securin; Tumor-transforming protein 1; unnamed protein product
Gene Aliases: AW555095; C87862; EAP1; ECRAR; HPTTG; PTTG; PTTG1; Pttg3; TUTR1
UniProt ID: (Human) O95997, (Mouse) Q9CQJ7
Entrez Gene ID: (Human) 9232, (Mouse) 30939
DNA repair
cellular response to DNA damage stimulus
chromosome segregation
spermatogenesis
homologous chromosome segregation
chromosome organization
cell division
regulation of cell growth
cell cycle
mitotic nuclear division
negative regulation of cell proliferation
cellular process
negative regulation of endopeptidase activity
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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