Search
Invitrogen
KLF5 Recombinant Rabbit Monoclonal Antibody (23H10L5)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 3
KLF5 Antibody (701885) in ICC/IF
For immunofluorescence analysis, A549 cells were fixed and permeabilized for detection of endogenous KLF5 using Anti- KLF5 Recombinant Rabbit Monoclonal Antibody (Product # 701885, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of KLF5 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938)... View More
Product Details
701885
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
23H10L5
Immunogen
Peptides corresponding to Human KLF5 (aa 11-24, 358-372)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Rabbit and Pig
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
KLF5 is a member of the Kruppel-like factor subfamily of zinc finger proteins. It contains three C2H2-type zinc fingers at the carboxyl-terminus that preferentially bind to cis-DNA elements that are GC rich. KLF5 is a transcription activator that promotes cell proliferation and oncogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: (intestinal Kruppel-like factor; basic transcription element binding protein 2; Basic transcription element-binding protein 2; BTE-binding protein 2; Colon krueppel-like factor; colon kruppel-like factor; epididymis secretory sperm binding protein; GC box binding protein 2; GC-box-binding protein 2; Intestinal-enriched krueppel-like factor; intestinal-enriched kruppel-like factor; Krueppel-like factor 5; Kruppel like factor 5; Kruppel-like factor 5 (intestinal); RP11-505F3.5; testis-specific transcript; tKLF5; transcription factor; Transcription factor BTEB2; unnamed protein product
Gene Aliases: BTEB2; CKLF; IKLF; KLF5
UniProt ID: (Human) Q13887
Entrez Gene ID: (Human) 688
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
MRF binding
sequence-specific DNA binding
metal ion binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
regulation of transcription from RNA polymerase II promoter
positive regulation of cell proliferation
regulation of gene expression
skeletal muscle satellite cell differentiation
satellite cell activation involved in skeletal muscle regeneration
myotube differentiation involved in skeletal muscle regeneration
microvillus assembly
regulation of microvillus assembly
skeletal muscle cell differentiation
skeletal muscle tissue regeneration
positive regulation of fat cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
intestinal epithelial cell development
positive regulation of transcription by transcription factor localization
cellular response to leukemia inhibitory factor
cellular response to endothelin
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support