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Invitrogen
CCR5 Recombinant Rabbit Monoclonal Antibody (2H14L10)
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FIGURE: 1 / 1
CCR5 Antibody (702233) in ICC/IF
For immunofluorescence analysis, RAW 264.7 cells were fixed and permeabilized for detection of endogenous CCR5 using Anti- CCR5 Recombinant Rabbit Monoclonal Antibody (Product # 702233, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of CCR5 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S3... View More
Product Details
702233
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
2H14L10
Immunogen
Peptides corresponding to Human CCR5 (aa 171-185, 333-347)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. The beta-chemokines RANTES, MIP-alpha and MIP-beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CCR5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C chemokine receptor type 5; C-C CKR-5; C-C motif chemokine receptor 5 A159A; CC-CKR-5; CCR-5; CD195; CD195 antigen; chemokine (C-C motif) receptor 5; chemokine (C-C) receptor 5; chemokine receptor CCR5; chemokine recptor CCR5 Delta32; CHEMR13; HIV-1 fusion coreceptor; MIP-1 alpha receptor; mutant C-C motif chemokine receptor; unnamed protein product
Gene Aliases: AM4-7; CC-CKR-5; CCCKR5; CCR-5; CCR5; CD195; CKR-5; CKR5; CMKBR5; IDDM22
UniProt ID: (Human) P51681, (Mouse) P51682
Entrez Gene ID: (Human) 1234, (Mouse) 12774
virus receptor activity
actin binding
phosphatidylinositol phospholipase C activity
G-protein coupled receptor activity
chemokine receptor activity
protein binding
coreceptor activity
C-C chemokine receptor activity
C-C chemokine binding
identical protein binding
chemokine (C-C motif) ligand 5 binding
glycoprotein binding
signal transducer activity
protein kinase binding
MAPK cascade
dendritic cell chemotaxis
calcium ion transport
apoptotic process
chemotaxis
defense response
inflammatory response
immune response
cellular defense response
signal transduction
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
cell-cell signaling
release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
calcium-mediated signaling
signaling
viral entry into host cell
cell chemotaxis
chemokine-mediated signaling pathway
response to cholesterol
cellular response to lipopolysaccharide
cellular response to cytokine stimulus
negative regulation of macrophage apoptotic process
positive regulation of gene expression
positive regulation of cell-cell adhesion
negative regulation of cell migration
negative regulation of axon extension
positive regulation of fever generation
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
positive regulation of neuron differentiation
positive regulation of interleukin-1 beta secretion
positive regulation of inflammatory response
positive regulation of apoptotic process by virus
neuron death
positive regulation of cell migration involved in sprouting angiogenesis
negative regulation of neural precursor cell proliferation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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