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          • Primary Antibodies ›
          • c-Abl Antibodies

          Invitrogen

          Phospho-c-Abl (Tyr393) Recombinant Rabbit Monoclonal Antibody (19H22L15)

          View all (58) c-Abl antibodies

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          Cite Phospho-c-Abl (Tyr393) Recombinant Rabbit Monoclonal Antibody (19H22L15)

          Additional Information:
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          • Antibody Testing Data (2)
          Phospho-c-Abl (Tyr393) Antibody in Immunocytochemistry (ICC/IF)
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          Phospho-c-Abl (Tyr393) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

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          Phospho-c-Abl (Tyr393) Antibody (702334) in ICC/IF

          For immunofluorescence analysis, K562 cells were fixed and permeabilized for detection of endogenous CAblpY393 Isoform A using Anti-CAblpY393 Isoform A Recombinant Rabbit Monoclonal Antibody (Product # 702334, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stain... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-c-Abl (Tyr393) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-c-Abl (Tyr393) Antibody in Western Blot (WB)
          Phospho-c-Abl (Tyr393) Recombinant Rabbit Monoclonal Antibody (19H22L15)

          Product Details

          702334

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Expression System

          Expi293

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          19H22L15

          Immunogen

          Peptide corresponding to Human ABL1 (aa 390-397)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Wet ice

          RRID

          AB_2632966

          Product Specific Information

          This antibody is predicted to react with Monkey, Horse, Dog and Bat

          Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

          Target Information

          The ABL1 proto-oncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns c-Abl into an oncogene. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for c-Abl. In chronic myelogenous leukemia and a subset of acute lymphoblastic leukemias, the c-Abl proto oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome). As the result of the fusion of c-Abl sequences from chromosome 9 to the Bcr gene on chromosome 22. The c-Abl oncogene was initially identified as the viral transforming gene of Abelson murine leukemia virus (A-MuLV). c-ABL potentially regulates DNA repair by activating the proapoptotic pathway when the DNA damage is too severe to be repaired.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: Abelson murine leukemia oncogene; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Abl 1 antib; ABL kinase domain; abl protein; ABL protooncogene 1 nonreceptor tyrosine kinase; ABL1; ABL2; ABLL; ARG; B30; BCR-ABL1 p190; BCR/ABL e8a2 fusion; BCR/ABL1 e1a2 fusion protein; BCR/ABL1 fusion; bcr/c-abl oncogene protein; c-abl oncogene 1, receptor tyrosine kinase; chimeric BCR::ABL1 protein; non-receptor tyrosine kinase; p150; p190; Proto-oncogene c-Abl; proto-oncogene tyrosine-protein kinase ABL1; RP11-83J21.1; Tyrosine kinase ARG; tyrosine kinase protein; Tyrosine-protein kinase ABL1; unnamed protein product; v-abl Abelson murine leukemia oncogene 1; v-abl Abelson murine leukemia viral oncogene homolog 1

          View more View less

          Gene Aliases: ABL; ABL1; AI325092; BCR-ABL; bcr/abl; c-ABL; c-ABL1; CHDSKM; E430008G22Rik; JTK7; p150; v-abl

          View more View less

          UniProt ID: (Human) P00519, (Mouse) P00520

          View more View less

          Entrez Gene ID: (Human) 25, (Mouse) 11350

          View more View less

          Function(s)
          nucleotide binding magnesium ion binding four-way junction DNA binding bubble DNA binding phosphotyrosine binding DNA binding transcription coactivator activity actin monomer binding nicotinate-nucleotide adenylyltransferase activity protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein kinase C binding protein binding ATP binding enzyme activator activity kinase activity transferase activity enzyme binding protein kinase binding protein domain specific binding syntaxin binding manganese ion binding neuropilin binding SH2 domain binding protein serine/threonine kinase activator activity metal ion binding ephrin receptor binding actin filament binding mitogen-activated protein kinase binding proline-rich region binding delta-catenin binding supercoiled DNA binding sequence-specific double-stranded DNA binding receptor binding protein C-terminus binding transferase activity, transferring phosphorus-containing groups SH3 domain binding
          Process(es)
          mitotic cell cycle neural tube closure B-1 B cell homeostasis immune effector process B cell proliferation involved in immune response transitional one stage B cell differentiation immune response-activating cell surface receptor signaling pathway DNA repair mismatch repair regulation of transcription, DNA-templated endocytosis phagocytosis autophagy apoptotic process cellular response to DNA damage stimulus response to oxidative stress cell adhesion epidermal growth factor receptor signaling pathway positive regulation of cytosolic calcium ion concentration integrin-mediated signaling pathway I-kappaB kinase/NF-kappaB signaling learning or memory associative learning intrinsic apoptotic signaling pathway in response to DNA damage response to xenobiotic stimulus post-embryonic development regulation of gene expression regulation of autophagy positive regulation of macromolecule biosynthetic process positive regulation of endothelial cell migration cerebellum morphogenesis negative regulation of cell-cell adhesion microspike assembly actin cytoskeleton organization actin filament polymerization regulation of endocytosis regulation of cell adhesion neuron differentiation BMP signaling pathway negative regulation of BMP signaling pathway regulation of axon extension phospholipase C-inhibiting G-protein coupled receptor signaling pathway regulation of microtubule polymerization regulation of Cdc42 protein signal transduction positive regulation of interferon-gamma production positive regulation of interleukin-2 production regulation of actin cytoskeleton organization cellular response to stress positive regulation of osteoblast proliferation substrate adhesion-dependent cell spreading cellular response to oxidative stress response to endoplasmic reticulum stress intracellular signal transduction platelet-derived growth factor receptor-beta signaling pathway protein modification process Fc-gamma receptor signaling pathway involved in phagocytosis neuropilin signaling pathway B cell proliferation regulation of cell proliferation signal transduction in response to DNA damage positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of neuron apoptotic process endothelial cell migration regulation of T cell differentiation regulation of cell differentiation positive regulation of cell adhesion positive regulation of vasoconstriction negative regulation of mitotic cell cycle positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter alpha-beta T cell differentiation platelet-derived growth factor receptor signaling pathway ephrin receptor signaling pathway positive regulation of fibroblast proliferation spleen development thymus development activated T cell proliferation T cell receptor signaling pathway B cell receptor signaling pathway neuromuscular process controlling balance positive regulation of release of sequestered calcium ion into cytosol neuron apoptotic process negative regulation of ubiquitin-protein transferase activity myoblast proliferation positive regulation of stress fiber assembly establishment of localization in cell regulation of cell cycle mitochondrial depolarization positive regulation of focal adhesion assembly Bergmann glial cell differentiation cardiac muscle cell proliferation neuroepithelial cell differentiation cellular response to hydrogen peroxide ERK1 and ERK2 cascade negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade DNA conformation change cellular response to lipopolysaccharide cellular response to transforming growth factor beta stimulus response to epinephrine circulatory system development positive regulation of cell migration involved in sprouting angiogenesis cellular senescence vascular endothelial cell response to oscillatory fluid shear stress cell-cell adhesion regulation of postsynaptic specialization assembly positive regulation of phospholipase C/protein kinase C signal transduction positive regulation of dendrite development positive regulation of substrate adhesion-dependent cell spreading negative regulation of long-term synaptic potentiation cellular response to oxygen-containing compound regulation of hematopoietic stem cell differentiation regulation of intracellular signal transduction regulation of extracellular matrix organization positive regulation of extracellular matrix organization glomerular visceral epithelial cell apoptotic process cellular response to dopamine positive regulation of establishment of T cell polarity DN4 thymocyte differentiation protein localization to cytoplasmic microtubule plus-end regulation of modification of synaptic structure positive regulation of blood vessel branching negative regulation of double-strand break repair via homologous recombination positive regulation of Wnt signaling pathway, planar cell polarity pathway regulation of cell motility negative regulation of endothelial cell apoptotic process positive regulation of T cell migration regulation of cellular senescence negative regulation of cellular senescence positive regulation of protein phosphorylation protein phosphorylation DNA damage induced protein phosphorylation phosphorylation cell migration peptidyl-tyrosine phosphorylation peptidyl-tyrosine autophosphorylation negative regulation of I-kappaB kinase/NF-kappaB signaling innate immune response collateral sprouting positive regulation of peptidyl-tyrosine phosphorylation positive regulation of oxidoreductase activity negative regulation of protein serine/threonine kinase activity actin filament branching positive regulation of interleukin-2 secretion negative regulation of phospholipase C activity positive regulation of neuron death positive regulation of interferon-gamma secretion regulation of response to DNA damage stimulus
          It has to be done as per old AB suggested Products section.

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