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Invitrogen
Phospho-CaMKII (Thr305,Thr306) Recombinant Rabbit Monoclonal Antibody (9H2L7)
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FIGURE: 1 / 2
Phospho-CaMKII (Thr305,Thr306) Antibody (702357) in ICC/IF
For immunofluorescence analysis, SH-SY5Y cells were fixed and permeabilized for detection of endogenous CamKIIpT305/306 using Anti- CamKIIpT305/306 Recombinant Rabbit Monoclonal Antibody (Product # 702357, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained f... View More
Product Details
702357
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
9H2L7
Immunogen
Peptide corresponding to Human CMK2A (aa 300-309)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Mouse, Dog, Sheep and Bovine
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
CAMK2A (CaMKII alpha) is a serine/threonine kinase that regulates a number of cellular functions including neuronal processes, gene expression, carbohydrate metabolism, cytoskeletal function, and calcium homeostatsis. Autophosphorylation of threonine 286 in the presence of Ca2+ and calmodulin activates CAMK2A with the ability to switch from calmodulin-dependent to a calmodulin independent activity. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+/calmodulin (CaM) binding and autophosphorylation and has been implicated in regulation of the cell cycle and transcription. It is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Calci; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CaM-kinase II alpha chain; CaMK-II alpha subunit; caMK-II subunit alpha; EC 2.7.11.17; OTTHUMP00000165787; OTTHUMP00000165788; Phospho-CaMKI; unnamed protein product
Gene Aliases: CAMK2A; CAMKA; CaMKIIalpha; CaMKIINalpha; KIAA0968; MRD53; MRT63
UniProt ID: (Human) Q9UQM7
Entrez Gene ID: (Human) 815
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
calmodulin-dependent protein kinase activity
protein binding
calmodulin binding
ATP binding
kinase activity
transferase activity
glutamate receptor binding
identical protein binding
protein homodimerization activity
metal ion binding
protein serine kinase activity
G1/S transition of mitotic cell cycle
response to ischemia
protein phosphorylation
calcium ion transport
JAK-STAT cascade
positive regulation of cardiac muscle cell apoptotic process
cellular response to interferon-beta
angiotensin-activated signaling pathway
positive regulation of JAK-STAT cascade
regulation of neurotransmitter secretion
regulation of neuronal synaptic plasticity
positive regulation of NF-kappaB transcription factor activity
negative regulation of hydrolase activity
positive regulation of calcium ion transport
long-term synaptic potentiation
dendritic spine development
cellular response to interferon-gamma
regulation of mitochondrial membrane permeability involved in apoptotic process
regulation of protein localization to plasma membrane
peptidyl-threonine autophosphorylation
regulation of endocannabinoid signaling pathway
regulation of neuron migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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