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Invitrogen
CHD8 Recombinant Rabbit Monoclonal Antibody (20H56L8)
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FIGURE: 1 / 4
CHD8 Antibody (703595) in ICC/IF
Knockdown of CHD8 was achieved by transfecting HCT 116 cells with specific siRNA (Silencer® select Product # S533282, S533284). Immunofluorescence analysis was performed on HCT 116 cells (untransfected, panel a-d), transfected with CHD8 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-CHD8 Recombinant Rabbit Monoclonal Antibody (Product # 703595, 1:100 dilution), followed by Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 48... View More
Product Details
703595
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
20H56L8
Immunogen
Protein corresponding to human CHD8 [aa2291-aa2364]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Mouse, Rat, Bovine
Target Information
DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent chromatin remodeler CHD8; ATP-dependent helicase CHD8; axis duplication inhibitor; CHD-8; Chromo domain-containing protein 8; duplin; helicase with SNF2 domain 1
Gene Aliases: AUTS18; CHD8; HELSNF1; IDDAM; KIAA1564
UniProt ID: (Human) Q9HCK8
Entrez Gene ID: (Human) 57680
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
startle response
chromatin organization
chromatin remodeling
mRNA processing
brain development
Wnt signaling pathway
negative regulation of Wnt signaling pathway
social behavior
negative regulation of apoptotic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase III promoter
digestive tract development
prepulse inhibition
negative regulation of canonical Wnt signaling pathway
negative regulation of fibroblast apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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