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IRAK1 Antibody (710015) in WB
Western blot analysis of IRAK1 was performed by loading 20 µg of HEK-293 (lane1), LNCaP (lane2), NIH/3T3 (lane3), HeLa (lane4), HT-29 (lane5), MCF7 (lane6) and K562 (lane7) cell lysate using Novex® NuPAGE®4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. IRAK1 ... View More
Product Details
710015
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
19HCLC
Immunogen
proprietary
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. It is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. IRAK's association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Alternatively, IRAK1 can phosphorylate TIRAP to promote its ubiquitination and subsequent degradation. IRAK1 also phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus. This results in the transcriptional activiation of type I IFN genes, which drive the cell in an antiviral state.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hIRAK1c; protein kinase; inactive protein kinase; signaling molecule; Interleukin-1 receptor-associated kinase 1; interleukin-1 receptor-associated kinase-1; IRAK; IRAK-1; mPLK; Pelle homolog; Pelle-like protein kinase
Gene Aliases: AA408924; Il1rak; IRAK; IRAK-1; IRAK1; IRAK1-S; mPLK; pelle; Plpk
UniProt ID: (Human) P51617, (Mouse) Q62406
Entrez Gene ID: (Human) 3654, (Mouse) 16179
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
protein kinase binding
heat shock protein binding
identical protein binding
protein homodimerization activity
protein heterodimerization activity
protein serine kinase activity
interleukin-1 receptor binding
transferase activity, transferring phosphorus-containing groups
regulation of cytokine-mediated signaling pathway
toll-like receptor signaling pathway
immune system process
MyD88-dependent toll-like receptor signaling pathway
regulation of transcription, DNA-templated
signal transduction
I-kappaB kinase/NF-kappaB signaling
JNK cascade
Toll signaling pathway
positive regulation of macromolecule metabolic process
cytokine-mediated signaling pathway
lipopolysaccharide-mediated signaling pathway
positive regulation of type I interferon production
response to lipopolysaccharide
toll-like receptor 2 signaling pathway
toll-like receptor 4 signaling pathway
toll-like receptor 9 signaling pathway
cellular response to heat
intracellular signal transduction
interleukin-33-mediated signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
protein autophosphorylation
positive regulation of smooth muscle cell proliferation
positive regulation of NF-kappaB transcription factor activity
type I interferon signaling pathway
interleukin-1-mediated signaling pathway
response to interleukin-1
cellular response to lipopolysaccharide
cellular response to hypoxia
positive regulation of intracellular signal transduction
positive regulation of leukocyte adhesion to vascular endothelial cell
protein phosphorylation
activation of NF-kappaB-inducing kinase activity
phosphorylation
negative regulation of NF-kappaB transcription factor activity
response to peptidoglycan
positive regulation of MAP kinase activity
positive regulation of JUN kinase activity
negative regulation of transcription, DNA-templated
protein oligomerization
negative regulation of cholesterol efflux
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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