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FIGURE: 1 / 5
Phospho-IKK alpha/beta (Ser176, Ser180) Antibody (710676) in ICC/IF
Time course showing induction of the TNF-a signaling cascade upon treatment: Cellular localization of proteins in the NF-KB signaling pathway was detected upon treatment of HeLa cells with TNF alpha (50 ng/mL) for 5, 10 and 25 min. Fixed and permeabilized cells were stained with Anti-IKK total Recombinant Rabbit Superclonal™ Antibody (Product # 710719, 1 µg/mL) or Anti-IKK alpha/beta (pS176/pS180) Recombina... View More
Product Details
710676
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
7HCLC
Immunogen
phosphopeptide corresponding to human IKK alpha (Ser176/180)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Dog, Rabbit, Pig, Mouse, Rat, Bovine and Goat.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
IKK alpha/beta is encoded by CHUK (conserved helix-loop-helix ubiquitous kinase) and IKKB (inhibitor of kappa light polypeptide gene enhancer in B-cells) gene located in chromosome 10 and 8 respectively in humans. Upon stimulation by agents that activate the NF-kB pathway such as TNF alpha, the two subunits of IKK complex, IKK alpha and IKK -beta alongside a regulator NEMO (IKK gamma) phosphorylates IkB proteins. This targets the later for ubiquitin mediated degradation and phosphorylation of NF-kB leading to subsequent translocation to the nucleus. Phosphorylation at serine 176 and 180 located in the activation loop causes a confirmation change that is required for the activation of the kinases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Conserved helix-loop-helix ubiquitous kinase; EC 2.7.11.10; I-kappa-B kinase 1; I-kappa-B kinase 2; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; I-kappa-B-kinase beta; IkappaB kinase; IkB kinase alpha subunit; IKK-1; IKK-2; IKK-a kinase; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; Inhibitor of nuclear factor kappa-B kinase subunit alpha; Inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKA; NFKBIKB; nuclear factor kappa B kinase subunit beta; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase beta; Nuclear factor NFkappaB inhibitor kinase alpha; protein kinase; Serine/threonine protein kinase IKBKB; TCF-16; TCF17; Transcription factor 16; unnamed protein product
Gene Aliases: BPS2; CHUK; IKBKA; IKBKB; IKK-1; IKK-2; IKK-alpha; IKK-beta; IKK1; IKK2; IKKA; IKKB; IMD15; IMD15A; IMD15B; NFKBIKA; NFKBIKB; TCF16
UniProt ID: (Human) O15111, (Human) O14920
Entrez Gene ID: (Human) 1147, (Human) 3551
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
IkappaB kinase activity
kinase activity
transferase activity
protein homodimerization activity
macromolecular complex binding
protein heterodimerization activity
scaffold protein binding
transferrin receptor binding
protein kinase binding
identical protein binding
protein serine kinase activity
pattern recognition receptor signaling pathway
skeletal muscle contraction
regulation of transcription from RNA polymerase II promoter
inflammatory response
immune response
I-kappaB kinase/NF-kappaB signaling
response to xenobiotic stimulus
response to virus
response to toxic substance
anatomical structure morphogenesis
response to acetate
negative regulation of NF-kappaB transcription factor activity
positive regulation of interferon-alpha production
response to hydroperoxide
tumor necrosis factor-mediated signaling pathway
toll-like receptor 4 signaling pathway
NIK/NF-kappaB signaling
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to amino acid
innate immune response
positive regulation of transcription from RNA polymerase II promoter
positive regulation of NF-kappaB transcription factor activity
striated muscle cell differentiation
response to cholecystokinin
cellular response to tumor necrosis factor
cellular response to virus
protein polyubiquitination
stimulatory C-type lectin receptor signaling pathway
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
MyD88-dependent toll-like receptor signaling pathway
protein phosphorylation
positive regulation of macromolecule metabolic process
regulation of tumor necrosis factor-mediated signaling pathway
peptidyl-serine phosphorylation
signal transduction involved in regulation of gene expression
regulation of toll-like receptor signaling pathway
toll-like receptor 3 signaling pathway
TRIF-dependent toll-like receptor signaling pathway
Fc-epsilon receptor signaling pathway
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription, DNA-templated
T cell receptor signaling pathway
stress-activated MAPK cascade
protein maturation
interleukin-1-mediated signaling pathway
protein localization to plasma membrane
negative regulation of cytokine production involved in inflammatory response
regulation of establishment of endothelial barrier
negative regulation of bicellular tight junction assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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