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FIGURE: 1 / 4
OTX2 Antibody (710981) in ICC/IF
Immunofluorescence was performed on fixed and permeabilized NTERA-2 cells for detection of OTX2 using Anti-OTX2 Recombinant Rabbit Superclonal™ Antibody (Product # 710981, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of OTX2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c... View More
Product Details
710981
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
14HCLC
Immunogen
Multiple peptides corresponding to Human OTX2 (aa 20-39; 276-289)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Rat, Mouse, Rabbit and Pig.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
OTX2 is a 289 amino acid containing protein encoding a member of the bicoid sub-family of homeodomain-containing transcription factors. It has two known alternative splice variants with distinct isoforms though presence of other isoforms cannot be excluded. The protein is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sensory organs. The protein may bind to BCD target sequence (BTS): 5-'TCTAATCCC-3'. Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: homeobox Otx2; Homeobox protein OTX2; nuclear protein; Orthodenticle homolog 2; OTX; unnamed protein product
Gene Aliases: CPHD6; E130306E05Rik; MCOPS5; OTX2
UniProt ID: (Human) P32243
Entrez Gene ID: (Human) 5015, (Rat) 305858, (Mouse) 18424
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
eukaryotic initiation factor 4E binding
sequence-specific double-stranded DNA binding
sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein heterodimerization activity
homeodomain transcription factor
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
axon guidance
regulation of smoothened signaling pathway
forebrain development
midbrain development
positive regulation of embryonic development
regulation of fibroblast growth factor receptor signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
protein-containing complex assembly
dopaminergic neuron differentiation
primitive streak formation
positive regulation of gastrulation
multicellular organism development
cell fate specification
transcription from RNA polymerase II promoter
protein complex assembly
central nervous system development
endoderm development
organ morphogenesis
anterior/posterior pattern specification
dorsal/ventral pattern formation
metencephalon development
cell differentiation
neuron differentiation
somite rostral/caudal axis specification
inner ear morphogenesis
eye photoreceptor cell fate commitment
cell fate commitment
neuron fate commitment
neuron fate determination
diencephalon morphogenesis
anatomical structure development
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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