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FIGURE: 1 / 2
TERT Antibody (711084) in ICC/IF
For immunofluorescence analysis, Hep G2 cells were fixed and permeabilized for detection of endogenous Telomerase reverse transcriptase using Anti- TERT Recombinant Rabbit Superclonal™ Antibody (Product # 711084, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of TERT protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mounta... View More
Product Details
711084
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
19HCLC
Immunogen
Peptides corresponding to Human TERT (aa 301-315, 643-657)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Dog and Bovine
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase (TERT) have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HEST2; hTERT; Telomerase catalytic subunit; Telomerase reverse transcriptase; Telomerase-associated protein 2; tert {ECO:0000312|RGD:70494}; TP2; unnamed protein product
Gene Aliases: CMM9; DKCA2; DKCB4; EST2; hEST2; hTRT; PFBMFT1; TCS1; TERT; TP2; TRT
UniProt ID: (Human) O14746, (Rat) Q673L6
Entrez Gene ID: (Human) 7015, (Rat) 301965
tRNA binding
transcription coactivator binding
DNA binding
telomerase activity
RNA binding
RNA-directed DNA polymerase activity
RNA-directed RNA polymerase activity
protein binding
transferase activity
nucleotidyltransferase activity
DNA polymerase activity
telomeric DNA binding
identical protein binding
protein homodimerization activity
metal ion binding
chaperone binding
telomerase RNA binding
template-free RNA nucleotidyltransferase
telomerase RNA reverse transcriptase activity
DNA metabolism protein
nucleic acid metabolism protein
telomere maintenance via recombination
telomere maintenance
transcription, RNA-templated
RNA-dependent DNA biosynthetic process
protein import into nucleus
telomere maintenance via telomerase
mitochondrion organization
heart development
DNA strand elongation
positive regulation of Wnt signaling pathway
production of siRNA involved in RNA interference
regulation of protein stability
positive regulation of hair cycle
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
positive regulation of angiogenesis
positive regulation of glucose import
response to cadmium ion
establishment of protein localization to telomere
cellular response to hypoxia
DNA biosynthetic process
replicative senescence
siRNA transcription
positive regulation of G1/S transition of mitotic cell cycle
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
positive regulation of transdifferentiation
positive regulation of vascular smooth muscle cell proliferation
positive regulation of protein localization to nucleolus
positive regulation of vascular associated smooth muscle cell migration
negative regulation of endothelial cell apoptotic process
positive regulation of stem cell proliferation
negative regulation of cellular senescence
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
obsolete age-dependent telomere shortening
biological_process
negative regulation of gene expression
telomere formation via telomerase
positive regulation of nitric-oxide synthase activity
negative regulation of production of siRNA involved in RNA interference
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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