Search
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
WNK1 Antibody (711356) in ICC/IF
For immunofluorescence analysis, A549 cells were fixed and permeabilized for detection of endogenous WNK1 using Anti- WNK1 Recombinant Rabbit Superclonal™ Antibody (Product # 711356, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of WNK1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938... View More
Product Details
711356
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
3HCLC
Immunogen
Peptides corresponding to Human WNK1 (aa 2338-2355, 45-56)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Rabbit, Pig and Mouse
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
WNK1 serine/threonine kinase is a member of the WNK subfamily. WNK1 may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. WNK plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Also, acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, and SGK1. WNK localizes to a segment of kidney involved in salt, potassium, and pH homeostasis, the distal nephron. Mutations in the WNK gene result in Pseudohypaldosteronism and Neuropathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Erythrocyte 65 kDa protein; hWNK1; KIAA0344; Kinase deficient protein; MGC163339; MGC163341; p65; prostate-derived sterile 20-like kinase; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; protein phosphatase 1, regulatory subunit 167; Serine/threonine-protein kinase WNK1; serine/threonine-protein kinase WNK1 1; serine/threonine-protein kinase WNK1 2; With no K
Gene Aliases: HSAN2; HSN2; KDP; KIAA0344; p65; PPP1R167; PRKWNK1; PSK; WNK1
UniProt ID: (Human) Q9H4A3
Entrez Gene ID: (Human) 65125
regulation of sodium ion transport
positive regulation of systemic arterial blood pressure
protein phosphorylation
ion transport
cell volume homeostasis
cellular response to DNA damage stimulus
signal transduction
heart development
regulation of blood pressure
negative regulation of autophagy
negative regulation of sodium ion transport
regulation of mRNA export from nucleus
positive regulation of T cell chemotaxis
cellular chloride ion homeostasis
negative regulation of protein ubiquitination
negative regulation of cell-cell adhesion mediated by integrin
negative regulation of heterotypic cell-cell adhesion
intracellular signal transduction
sodium ion transmembrane transport
chemokine (C-C motif) ligand 21 signaling pathway
homeostatic process
protein insertion into ER membrane by stop-transfer membrane-anchor sequence
positive regulation of angiogenesis
neuron development
T cell receptor signaling pathway
negative regulation of small GTPase mediated signal transduction
potassium ion homeostasis
cation homeostasis
cellular hyperosmotic response
potassium ion transmembrane transport
negative regulation of pancreatic juice secretion
positive regulation of canonical Wnt signaling pathway
lymphocyte migration into lymph node
membraneless organelle assembly
regulation of sodium ion transmembrane transport
negative regulation of leukocyte cell-cell adhesion
negative regulation of protein localization to plasma membrane
positive regulation of mitotic cytokinesis
regulation of cation transmembrane transport
positive regulation of termination of RNA polymerase II transcription
cellular response to chemokine
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support