Search
Additional Information:
{{banner.description}}
FIGURE: 1 / 3
ANP Antibody (711569) in WB
Western blot analysis was performed on Tissue extracts (30 µg lysate) of Mouse heart (Lane 1), Rat heart (Lane 2), Mouse Brain (Lane 3) and Rat brain (Lane 4). The blots were probed with Anti-NPPA (ANP) Recombinant Rabbit Superclonal™ Antibody (Product # 711569, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 17 kDa band corresponding to NPPA (ANP) was observed only in Heart tissue extracts tested. Known qua... View More
Product Details
711569
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
17 HCLC
Immunogen
Peptide corresponding to human ANP (aa127- 145)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Pig
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: atrial natriuretic factor; Atrial natriuretic factor prohormone; atrial natriuretic peptide; Atrial natriuretic peptide prohormone; Atriopeptigen; atriopeptin; Cardiodilatin; cardiodilatin-related peptide; cardionatrin; CDD; natriuretic peptide precursor A; Natriuretic peptide precursor A (pronatriodilatin, also Anf, Pnd); natriuretic peptide precursor A variant NPPA-M1; natriuretic peptide precursor A variant NPPA-M2; natriuretic peptide precursor A variant NPPA-M3; natriuretic peptide precursor type A; Natriuretic peptides A; preproANF; preproANP; preprocardiodilatin-atrial natriuretic factor; preproCDD-ANF; prepronatiodilatin (PND); prepronatriodilatin; proANF; proANP; Pronatriodilatin; urodilatin
Gene Aliases: ANF; ANP; ATFB6; ATRST2; CDD; CDD-ANF; CDP; NPPA; PND; RATANF
UniProt ID: (Human) P01160, (Rat) P01161, (Mouse) P05125
Entrez Gene ID: (Human) 4878, (Rat) 24602, (Mouse) 230899
response to hypoxia
system process
negative regulation of systemic arterial blood pressure
cardiac conduction system development
aortic valve morphogenesis
cGMP biosynthetic process
protein folding
receptor guanylyl cyclase signaling pathway
neuropeptide signaling pathway
female pregnancy
regulation of blood pressure
positive regulation of heart rate
positive regulation of cGMP-mediated signaling
cardiac muscle hypertrophy in response to stress
cGMP-mediated signaling
negative regulation of cell growth
response to insulin
response to muscle stretch
sodium ion export from cell
negative regulation of epidermal growth factor receptor signaling pathway
vasodilation
regulation of vascular permeability
negative regulation of JUN kinase activity
negative regulation of blood pressure
regulation of atrial cardiac muscle cell membrane repolarization
positive regulation of cardiac muscle contraction
cell growth involved in cardiac muscle cell development
cellular response to hydrogen peroxide
cellular response to mechanical stimulus
negative regulation of canonical Wnt signaling pathway
regulation of blood vessel diameter
synaptic signaling via neuropeptide
regulation of calcium ion transmembrane transport via high voltage-gated calcium channel
positive regulation of potassium ion export across plasma membrane
negative regulation of collecting lymphatic vessel constriction
cellular response to angiotensin
response to 3-methylcholanthrene
heart development
regulation of body fluid levels
regulation of blood vessel size
multicellular organismal water homeostasis
regulation of high voltage-gated calcium channel activity
positive regulation of delayed rectifier potassium channel activity
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support