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FIGURE: 1 / 4
Phospho-PKA alpha/beta (Thr197) Antibody (711615) in ICC/IF
For immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of PKA CAT ALPHA/BETA (pT197) using Anti-PKA CAT ALPHA/BETA (pT197) Recombinant Rabbit Superclonal™ Antibody (Product # 711615, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were s... View More
Product Details
711615
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
24HCLC
Immunogen
Peptide corresponding to Human RSK1, RSK2, RSK3, RSK4 (Chemically synthesized phosphopeptide derived from a region of human PKA that contains threonine 197.)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Human, Mouse, Monkey, Pig and Bovine
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PRKACB is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-alpha-s; alternatively spliced isoform; cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit beta; PKA C-alpha; PKA C-beta; protein kinase A catalytic subunit; protein kinase A catalytic subunit beta; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, beta catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha; protein kinase, cAMP-dependent, catalytic, beta; unnamed protein product
Gene Aliases: CAFD1; CAFD2; PKA C-beta; PKACA; PKACB; PPNAD4; PRKACA; PRKACB
UniProt ID: (Human) P17612, (Human) P22694
Entrez Gene ID: (Human) 5566, (Human) 5567
nucleotide binding
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
cAMP-dependent protein kinase activity
protein serine/threonine/tyrosine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
protein kinase binding
protein domain specific binding
manganese ion binding
ubiquitin protein ligase binding
protein kinase A regulatory subunit binding
channel activator activity
protein serine kinase activity
mesoderm formation
neural tube closure
regulation of heart rate
renal water homeostasis
mRNA processing
protein phosphorylation
protein export from nucleus
adenylate cyclase-activating G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
regulation of macroautophagy
peptidyl-serine phosphorylation
cytokine-mediated signaling pathway
cellular potassium ion homeostasis
cellular response to nutrient levels
positive regulation of insulin secretion
negative regulation of interleukin-2 production
high-density lipoprotein particle assembly
cellular response to heat
mitochondrial protein catabolic process
interleukin-2-mediated signaling pathway
TORC1 signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of cholesterol biosynthetic process
regulation of osteoblast differentiation
positive regulation of gluconeogenesis
negative regulation of smoothened signaling pathway
positive regulation of protein export from nucleus
sperm capacitation
positive regulation of phagocytosis
modulation of synaptic transmission
positive regulation of calcium-mediated signaling
regulation of cell cycle
regulation of cardiac muscle contraction
regulation of proteasomal protein catabolic process
cellular response to cold
regulation of protein processing
cellular response to glucose stimulus
cellular response to parathyroid hormone stimulus
cellular response to glucagon stimulus
cellular response to epinephrine stimulus
cell communication by electrical coupling involved in cardiac conduction
vascular endothelial cell response to laminar fluid shear stress
postsynaptic modulation of chemical synaptic transmission
cAMP/PKA signal transduction
regulation of cardiac conduction
negative regulation of TORC1 signaling
negative regulation of glycolytic process through fructose-6-phosphate
protein localization to lipid particle
regulation of bicellular tight junction assembly
signal transduction
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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