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Invitrogen
PKN1 Polyclonal Antibody
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FIGURE: 1 / 4
PKN1 Antibody (720396) in ICC/IF
For immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of endogenous PKN1 using Anti- PKN1 Rabbit Polyclonal Antibody (Product # 720396, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of PKN1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) r... View More
Product Details
720396
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptides corresponding to human PKN1 [1) 4-22 2) aa922-aa939]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 30% glycerol
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Bovine, Dog.
Target Information
PKN1 (PRK1) belongs to the protein kinase C superfamily. This kinase is activated by the Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can also be activated by phospholipids. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PAK-1; Protease-activated kinase 1; Protein kinase C-like 1; Protein kinase C-like PKN; protein kinase C-related kinase 1; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; serine-threonine kinase N; Serine-threonine protein kinase N; serine/threonine protein kinase N; Serine/threonine-protein kinase N1; unnamed protein product
Gene Aliases: DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PKN1; PRK1; PRKCL1
UniProt ID: (Human) Q16512
Entrez Gene ID: (Human) 5585
nucleotide binding
chromatin binding
transcription coactivator activity
protein kinase activity
protein serine/threonine kinase activity
protein kinase C activity
protein kinase C binding
protein binding
ATP binding
kinase activity
transferase activity
small GTPase binding
histone kinase activity (H3-T11 specific)
histone binding
histone deacetylase binding
androgen receptor binding
protein serine kinase activity
B cell homeostasis
B cell apoptotic process
regulation of germinal center formation
regulation of immunoglobulin production
renal system process
chromatin organization
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
protein phosphorylation
hyperosmotic response
signal transduction
epithelial cell migration
negative regulation of B cell proliferation
intracellular signal transduction
post-translational protein modification
positive regulation of transcription, DNA-templated
spleen development
regulation of androgen receptor signaling pathway
regulation of cell motility
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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