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Invitrogen
Goat anti-Human IgG Fc Highly Cross-Adsorbed Secondary Antibody
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FIGURE: 1 / 2
Human IgG Fc Highly Cross-Adsorbed Secondary Antibody (A18831) in WB
Western blot (non-reducing) was performed using Goat anti-Human IgG Fc Highly Cross-Adsorbed Secondary Antibody (Product # A18831) and a 150 kDa band corresponding toHuman IgG was observed in IM-9, ARH-77 and IM-9, ARH-77 conditioned medium8203(CM) but not in Raji,Jurkat, Molt-4 andJurkatCM which are known to have lowexpression. Whole cell lysate (30 µg) of IM-9 (Lane 1), ARH-77 (Lane 2), Raji (Lane 3), Jur... View More
Product Details
A18831
Applications
Tested Dilution
Western Blot (WB)
1:2,000
ELISA (ELISA)
1:500-1:20,000
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Gamma Immunoglobin Fc region
Conjugate
Form
Liquid
Concentration
> 4.5 mg/mL
Amount
1 mg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG Fc
Cross Adsorption
Against bovine, horse, mouse and rabbit serum proteins, as well as human IgA and IgM
Antibody Form
Whole Antibody
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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