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Invitrogen
F(ab')2-Goat anti-Human IgM (Heavy chain) Secondary Antibody
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FIGURE: 1 / 4
Human IgM (Heavy chain) Secondary Antibody (A24484) in ICC/IF
Immunofluorescence analysis of F(ab)2-Goat anti-Human IgM (Heavy chain) Secondary Antibody was performed using log phaseRamos cells (IgM producing B-cell line). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with0.1% Triton™ X-100 for 15minutes, andblocked with 2% BSA for 1 hour at room temperature. The cells were labeled with F(ab)2-Goat anti-Human IgM (Heavy chain) Secondary A... View More
Product Details
A24484
Applications
Tested Dilution
Western Blot (WB)
1:2000
Immunocytochemistry (ICC/IF)
1:250
ELISA (ELISA)
1:500-1:20,000
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Human Mu immunoglobin
Conjugate
Form
Liquid
Concentration
> 4.5 mg/mL
Amount
1 mg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.2
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgM (Heavy chain)
Antibody Form
F(ab')2 Fragment
Product Specific Information
The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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